Hajeer A H, Balfour A H, Mostratos A, Crosse B
ARC Unit, Medical School, Manchester, UK.
Parasite Immunol. 1994 Jan;16(1):43-50. doi: 10.1111/j.1365-3024.1994.tb00303.x.
Saliva samples from 27 patients with a recent toxoplasma infection were tested for specific IgG, IgM and IgA antibodies to Toxoplasma gondii. Thirteen of the 27 saliva samples were positive for IgG anti-T. gondii by direct agglutination and 8 of the 27 were positive for IgM anti-T. gondii by an immunosorbent agglutination assay. Twenty of the 27 saliva samples were positive for IgG antibody on toxoplasma immunoblots with three major immunodominant antigens; 38, 30 and 35 kDa. IgA results on toxoplasma immunoblots were positive for all three groups tested, recently infected patients, chronically infected and seronegative adults without distinguishing between them. The 35 and 43 kDa antigens were the most frequently detected proteins. IgM in saliva gave negative or very weak reactions. None of the eight seronegative or the 17 chronically infected adults gave positive results in any of the tests performed to detect IgG or IgM in saliva. Serial saliva and serum samples from a laboratory-infected patient were collected and tested for toxoplasma-specific IgG, IgM and IgA. IgG in saliva was detected by 27 days post infection (p.i.) and was negative by 81 days p.i.; it detected mainly the 38 and 30 kDa antigens. IgM in saliva was detected by 11 days p.i. and was negative by 81 days p.i., with no reaction on immunoblots.
对27例近期感染弓形虫的患者的唾液样本进行检测，以检测针对刚地弓形虫的特异性IgG、IgM和IgA抗体。27份唾液样本中有13份通过直接凝集法检测到抗弓形虫IgG呈阳性，27份中有8份通过免疫吸附凝集试验检测到抗弓形虫IgM呈阳性。27份唾液样本中有20份在针对三种主要免疫显性抗原（38、30和35 kDa）的弓形虫免疫印迹上IgG抗体呈阳性。在弓形虫免疫印迹上，IgA结果在所有检测的三组中均呈阳性，包括近期感染患者、慢性感染患者和血清阴性的成年人，未对他们进行区分。35和43 kDa抗原是最常检测到的蛋白质。唾液中的IgM呈阴性或反应非常微弱。在检测唾液中IgG或IgM的任何一项检测中，8例血清阴性或17例慢性感染的成年人均未得出阳性结果。收集了一名实验室感染患者的系列唾液和血清样本，并检测了弓形虫特异性IgG、IgM和IgA。感染后27天在唾液中检测到IgG，感染后81天呈阴性；它主要检测到38和30 kDa抗原。感染后11天在唾液中检测到IgM，感染后81天呈阴性，在免疫印迹上无反应。
