Department of Chemistry, Stanford University, William Keck Science Building rm 125, Stanford, CA, 94305-5080, USA.
Service de Parasitologie Mycologie, Centre Hospitalier Universitaire de Nice, INSERM, U1065, Centre Méditerranéen de Médecine Moléculaire, Faculté de Médecine, Virulence microbienne et signalisation inflammatoire - Université de la Côte d'Azur, CS 23079, Nice, cedex 3, France.
Eur J Clin Microbiol Infect Dis. 2019 May;38(5):883-890. doi: 10.1007/s10096-019-03487-1. Epub 2019 Jan 30.
Sampling the blood compartment by an invasive procedure such as phlebotomy is the most common approach used for diagnostic purposes. However, phlebotomy has several drawbacks including pain, vasovagal reactions, and anxiety. Therefore, alternative approaches should be tested to minimize patient's discomfort. Saliva is a reasonable compartment; when obtained, it generates little or no anxiety. We setup a multiplexed serology assay for detection of Toxoplasma gondii IgG and IgM, rubella IgG, and CMV IgG, in serum, whole blood, and saliva using novel plasmonic gold (pGOLD) chips. pGOLD test results in serum, whole blood, and saliva were compared with commercial kits test results in serum. One hundred twenty serum/saliva sets (Lyon) and 28 serum/whole blood/saliva sets (Nice) from France were tested. In serum and whole blood, sensitivity and specificity of multiplex T. gondii, CMV, and rubella IgG were 100% in pGOLD when compared to commercial test results in serum. In saliva, sensitivity and specificity for T. gondii and rubella IgG were 100%, and for CMV IgG, sensitivity and specificity were 92.9% and 100%, respectively, when compared to commercial test results in serum. We were also able to detect T. gondii IgM in saliva with sensitivity and specificity of 100% and 95.4%, respectively, when compared to serum test results. Serological testing by multiplex pGOLD assay for T. gondii, rubella, and CMV in saliva is reliable and likely to be more acceptable for systematic screening of pregnant women, newborn, and immunocompromised patients.
通过侵入性程序（如静脉穿刺）从血液腔室采样是用于诊断目的的最常见方法。然而，静脉穿刺有几个缺点，包括疼痛、血管迷走神经反应和焦虑。因此，应该测试替代方法以最大程度地减少患者的不适。唾液是一个合理的腔室；当获得时，它会产生很少或没有焦虑。我们使用新型等离子体金（pGOLD）芯片在血清、全血和唾液中建立了用于检测弓形体 IgG 和 IgM、风疹 IgG 和 CMV IgG 的多重血清学检测方法。pGOLD 测试在血清、全血和唾液中的结果与血清中的商业试剂盒测试结果进行了比较。我们对来自法国的 120 份血清/唾液组（里昂）和 28 份血清/全血/唾液组（尼斯）进行了测试。在血清和全血中，与血清中的商业检测结果相比，pGOLD 中多重 T. gondii、CMV 和风疹 IgG 的敏感性和特异性均为 100%。在唾液中，T. gondii 和风疹 IgG 的敏感性和特异性均为 100%，CMV IgG 的敏感性和特异性分别为 92.9%和 100%。与血清检测结果相比，我们还能够在唾液中检测到 T. gondii IgM，其敏感性和特异性分别为 100%和 95.4%。通过 pGOLD 多重检测方法在唾液中对 T. gondii、风疹和 CMV 进行血清学检测是可靠的，并且可能更适合对孕妇、新生儿和免疫功能低下患者进行系统筛查。
