Zhen L X, Ishii K, Taketa K, Ogata M
Department of Public Health, Okayama University Medical School, Japan.
Physiol Chem Phys Med NMR. 1993;25(4):253-60.
The inhibitory effect of alpha-tocopherol on methemoglobin formation in normal and acatalasemic mice was studied by exposing their hemolysates to nitric oxide. Methemoglobin formation in normal and acatalasemic mouse hemolysates exposed to nitric oxide were significantly inhibited by the addition of alpha-tocopherol at final concentrations ranging from 1.2 to 5.8 mM. Negative correlations were observed between the logarithm of alpha-tocopherol concentration and the methemoglobin formation. The formation of methemoglobin in acatalasemic mouse hemolysates was greater than that in normal mouse hemolysates with or without added alpha-tocopherol. The methemoglobin formation in acatalasemic mice was also significantly inhibited by addition of more than 500 units/ml of catalase, and the methemoglobin formation in normal and acatalasemic mice was also inhibited with sodium diethyldithiocarbamate at a final concentration of 1 M.
通过将正常小鼠和无过氧化氢酶小鼠的溶血产物暴露于一氧化氮，研究了α-生育酚对高铁血红蛋白形成的抑制作用。在最终浓度为1.2至5.8 mM的α-生育酚添加情况下，暴露于一氧化氮的正常小鼠和无过氧化氢酶小鼠的溶血产物中的高铁血红蛋白形成受到显著抑制。观察到α-生育酚浓度的对数与高铁血红蛋白形成之间呈负相关。在添加或未添加α-生育酚的情况下，无过氧化氢酶小鼠溶血产物中的高铁血红蛋白形成均高于正常小鼠溶血产物中的高铁血红蛋白形成。添加超过500单位/毫升的过氧化氢酶也可显著抑制无过氧化氢酶小鼠中的高铁血红蛋白形成，并且最终浓度为1 M的二乙基二硫代氨基甲酸钠也可抑制正常小鼠和无过氧化氢酶小鼠中的高铁血红蛋白形成。
