Richards R M, Cavill R H
J Pharm Sci. 1976 Jan;65(1):76-80. doi: 10.1002/jps.2600650115.
Electron micrographs of Pseudomonas aeruginosa grown in nutrient broth or broth containing subinhibitory concentrations of benzalkonium chloride indicate that benzalkonium chloride at 50 and 100 mug/ml strips off the outer cell membrane. Cells grown in the presence of edetate disodium, 50-100 mug/ml had convoluted surfaces (wavy in cross section). Cells damaged by growing on nutrient agar containing benzalkonium (200 mug/ml), when subsequently grown for 16 hr in nutrient broth, produced cells with apparently normal outer lavers. Similar cells grown on nutrient agar plus benzalkonium (500 mug/ml) when grown for 16 hr in nutrient broth had normal resistance to edetate disodium lysis, but cells grown overnight in broth plus benzalkonium (500 mug/ml) showed increased sensitivity to edetate disodium lysis. Cells grown on nutrient agar in the presence of benzalkonium (800 mug/ml) grew in broth plus benzalkonium (10 mug/ml) without stripping of their external membrane, but replicate inocula into broth plus benzalkonium (10 mug/ml) and edetate disodium (50 mug/ml) produced cells with structural damage to the outer lavers of the cell envelope.
在营养肉汤或含有亚抑制浓度苯扎氯铵的肉汤中生长的铜绿假单胞菌的电子显微镜照片表明，50和100微克/毫升的苯扎氯铵会剥离细胞外膜。在50 - 100微克/毫升乙二胺四乙酸二钠存在下生长的细胞表面呈卷曲状（横截面呈波浪状）。在含有苯扎氯铵（200微克/毫升）的营养琼脂上生长而受损的细胞，随后在营养肉汤中培养16小时后，产生的细胞外膜明显正常。在营养琼脂加苯扎氯铵（500微克/毫升）上生长的类似细胞，在营养肉汤中培养16小时后，对乙二胺四乙酸二钠裂解具有正常抗性，但在肉汤加苯扎氯铵（500微克/毫升）中过夜培养的细胞对乙二胺四乙酸二钠裂解的敏感性增加。在苯扎氯铵（800微克/毫升）存在下在营养琼脂上生长的细胞，在肉汤加苯扎氯铵（10微克/毫升）中生长时其外膜不会被剥离，但将重复接种物接种到肉汤加苯扎氯铵（10微克/毫升）和乙二胺四乙酸二钠（50微克/毫升）中会产生细胞包膜外层结构受损的细胞。
