Farrington M, McGinnes J, Matthews I, Park G R
Intensive Care Unit, Addenbrooke's NHS Trust, Cambridge.
Br J Anaesth. 1994 Apr;72(4):415-7. doi: 10.1093/bja/72.4.415.
In order to investigate bacterial contamination of i.v. anaesthetic agents, given by infusion to critically ill patients, we have cultured residual infusion fluid from infusion syringes, 50 containing midazolam and 50 propofol. The infusions had been prepared with routine aseptic precautions and had been running for between 0.75 and 21.25 h. Only scanty growths of Staphylococcus epidermidis were isolated from seven syringes (four midazolam and three propofol). Small volume samples were more likely to produce bacterial growth than large volume specimens. Midazolam infusions made up in 5% glucose were more likely to be contaminated than those made up in 0.9% saline. Antibacterial activity was detected in 18 midazolam and one propofol filtrate. Midazolam infusions inhibited the growth of all seven of the S. epidermidis isolates, whereas propofol supported similar rates of multiplication to that obtained with control broth medium. The results of this study imply that contamination of the infusions probably occurred after they were disconnected from the patient. Despite the ability of propofol to support microbial multiplication, we have no evidence to suggest that this is clinically significant when infusions are prepared with conventional aseptic precautions.
为调查重症患者静脉输注麻醉剂时的细菌污染情况，我们对50支含咪达唑仑和50支含丙泊酚的输液注射器中的残留输液液进行了培养。输液是在常规无菌预防措施下配制的，输注时间为0.75至21.25小时。仅从7支注射器（4支咪达唑仑和3支丙泊酚）中分离出少量表皮葡萄球菌。小体积样本比大体积样本更易产生细菌生长。用5%葡萄糖配制的咪达唑仑输液比用0.9%盐水配制的更易被污染。在18份咪达唑仑滤液和1份丙泊酚滤液中检测到抗菌活性。咪达唑仑输液抑制了所有7株表皮葡萄球菌分离株的生长，而丙泊酚支持的细菌繁殖速率与对照肉汤培养基相似。本研究结果表明，输液污染可能发生在与患者断开连接之后。尽管丙泊酚有支持微生物繁殖的能力，但我们没有证据表明在采用常规无菌预防措施配制输液时这具有临床意义。
