van der Zee H
Inspectorate for Health Protection Amsterdam, The Netherlands.
Int J Food Microbiol. 1994 Jan;21(1-2):41-6. doi: 10.1016/0168-1605(94)90198-8.
Conventional methods for the specific isolation of Salmonella enteritidis are scarce. For pre-enrichment, addition of ammonium-iron (III)-citrate, ferrioxamine E and G or novobiocin in combination with cefsoludin to Buffered Peptone Water (BPW) and of ferrous sulphate to Trypticase Soy Broth seems to favour S. enteritidis isolation. As far as selective media are concerned, the use of semi-solid media, and addition of nitrofurantoin, results in higher isolation rates of the S. enteritidis serovar. Addition of 0.0015% nitrofurantoin to semi-solid DIASALM is so far the only successful combination reported. Addition of 0.0015% nitrofurantoin to solid media, in this case to XLD, is also reported. Use of a semi-solid medium, preferably DIASALM + 0.0015% nitrofurantoin, in addition to the selective media routinely used is recommended.
用于特异性分离肠炎沙门氏菌的传统方法很少。对于预富集，在缓冲蛋白胨水（BPW）中添加柠檬酸铁（III）铵、去铁胺E和G或新生霉素与头孢磺啶联用，以及在胰蛋白胨大豆肉汤中添加硫酸亚铁似乎有利于肠炎沙门氏菌的分离。就选择性培养基而言，使用半固体培养基以及添加呋喃妥因可提高肠炎沙门氏菌血清型的分离率。迄今为止，向半固体DIASALM中添加0.0015%呋喃妥因是唯一报道成功的组合。也有报道称向固体培养基（如XLD）中添加0.0015%呋喃妥因。除常规使用的选择性培养基外，建议使用半固体培养基，最好是DIASALM + 0.0015%呋喃妥因。
