等离子体诱导的聚甲基丙烯酸羟乙酯（PHEMA）接枝共聚对硅橡胶改善角膜上皮细胞生长的影响。

The effect of plasma-induced graft copolymerization of PHEMA on silicone rubber towards improving corneal epithelial cells growth.

作者信息

Hsiue G H, Lee S D, Wang C C, Chang P C

机构信息

Department of Chemical Engineering, National Tsing Hua University, Hsinchu, Taiwan, Republic of China.

出版信息

J Biomater Sci Polym Ed. 1993;5(3):205-20. doi: 10.1163/156856293x00302.

DOI:10.1163/156856293x00302

PMID:8155609

Abstract

A PHEMA grafted polymer film was prepared by plasma induced graft copolymerization onto an elastic material, silicone rubber. The control, Ar plasma-treated, and PHEMA-grafted silicone rubber surfaces were characterized by ESCA, FTIR-ATR, and SEM techniques. ESCA verified the respective chemical shift of control and Ar plasma-treated films. The presence of the grafted PHEMA was also verified by ESCA. The amounts of grafted PHEMA did not monotonously increase with the plasma exposure conditions, but decreased after passing a maximum. The introduction of PHEMA onto a hydrophobic support provided an adequate surface for rabbit corneal epithelium cell attachment and growth. Cell attachment and growth onto these surfaces were examined by light microscopy. Cell attachment onto the control and Ar plasma-treated surface was negligible, while improved attachment and growth of rabbit corneal epithelium cells was demonstrated on the PHEMA-grafted polymer surface. The PHEMA-grafted silicone rubber surface demonstrated a confluent cell layer after 72 h.

摘要

通过等离子体诱导接枝共聚法在弹性材料硅橡胶上制备了聚甲基丙烯酸羟乙酯（PHEMA）接枝聚合物薄膜。采用光电子能谱（ESCA）、傅里叶变换红外光谱衰减全反射（FTIR-ATR）和扫描电子显微镜（SEM）技术对对照样、氩等离子体处理样和PHEMA接枝硅橡胶表面进行了表征。ESCA验证了对照样和氩等离子体处理薄膜各自的化学位移。ESCA也证实了接枝PHEMA的存在。接枝PHEMA的量并非随等离子体暴露条件单调增加，而是在达到最大值后下降。将PHEMA引入疏水载体上为兔角膜上皮细胞的附着和生长提供了合适的表面。通过光学显微镜检查细胞在这些表面上的附着和生长情况。细胞在对照样和氩等离子体处理表面上的附着可以忽略不计，而在PHEMA接枝聚合物表面上则显示出兔角膜上皮细胞附着和生长得到改善。PHEMA接枝硅橡胶表面在72小时后显示出汇合的细胞层。