用于改善兔角膜上皮细胞附着和生长的聚甲基丙烯酸羟乙酯改性硅橡胶膜

ppHEMA-modified silicone rubber film towards improving rabbit corneal epithelial cell attachment and growth.

作者信息

Hsiue G H, Lee S D, Wang C C, Shiue M H, Chang P C

机构信息

Department of Chemical Engineering, National Tsing Hua University, Hsinchu, Taiwan ROC.

出版信息

Biomaterials. 1993 Jul;14(8):591-7. doi: 10.1016/0142-9612(93)90177-4.

DOI:10.1016/0142-9612(93)90177-4

PMID:8399952

Abstract

A plasma polymerized HEMA (ppHEMA) film was prepared by plasma deposition polymerization onto an elastic material, silicone rubber. The surfaces of control, argon plasma-treated, and ppHEMA-modified silicone rubber were characterized by ESCA, FTIR-ATR and SEM techniques. ESCA verified the respective chemical shift of control and ppHEMA-modified films. The presence of the ppHEMA was also verified by ESCA. The introduction of ppHEMA onto a hydrophobic support provided an adequate surface for rabbit corneal epithelial cell attachment and growth. Cell attachment and growth onto these surfaces were examined by light microscopy. Cell attachment onto the control and the argon plasma-treated surface was negligible, while improved attachment and growth of rabbit corneal epithelium cells was demonstrated on the ppHEMA-modified polymeric surface. The ppHEMA-modified silicone rubber surface demonstrated a confluent cell layer after 72 h.

摘要

通过等离子体沉积聚合在弹性材料硅橡胶上制备了等离子体聚合甲基丙烯酸羟乙酯（ppHEMA）薄膜。采用光电子能谱（ESCA）、傅里叶变换红外衰减全反射光谱（FTIR-ATR）和扫描电子显微镜（SEM）技术对对照、氩等离子体处理和ppHEMA改性的硅橡胶表面进行了表征。ESCA验证了对照膜和ppHEMA改性膜各自的化学位移。ESCA也证实了ppHEMA的存在。将ppHEMA引入疏水载体上为兔角膜上皮细胞的附着和生长提供了合适的表面。通过光学显微镜检查细胞在这些表面上的附着和生长情况。细胞在对照表面和氩等离子体处理表面上的附着可以忽略不计，而在ppHEMA改性的聚合物表面上，兔角膜上皮细胞的附着和生长得到了改善。72小时后，ppHEMA改性的硅橡胶表面呈现出汇合的细胞层。