Veldman J E, Keuning F J, Molenaar I
Virchows Arch B Cell Pathol. 1978 Oct 16;28(3):187-202. doi: 10.1007/BF02889069.
Two times sublethal total body-X-irradiation with weekly local thymus irradiation established a T-cell deprived experimental model in rabbits. Humoral immunity reactions in draining lymph nodes have been analyzed histologically and at the submicroscopical level after challenge with Salmonella Java vaccine, horse spleen ferritin, horse-gamma-globulin, a chemical sensitizer oxazolone (2 phenyl-4-ethoxymethylene-5-oxazolone) and after skin allografting respectively. The time sequence studies in these animals with an 'isolated B-cell system' are compared with similar experiments in normal non-irradiated rabbits. The site of initiation of the thymus-independent and thymus-dependent plasma cell response is established in the lymph node. The (ultra)structural features of the antibody forming (-B-)cell precursors, the marginal zone cells, are described and discussed. The differentiating off-spring of two (sub)microscopically recognizable plasma cell lines is presented.
每周对局部胸腺进行一次照射，同时对全身进行两次亚致死剂量的X射线照射，从而建立了兔T细胞缺乏的实验模型。在用爪哇沙门氏菌疫苗、马脾铁蛋白、马γ球蛋白、化学致敏剂恶唑酮（2-苯基-4-乙氧基亚甲基-5-恶唑酮）进行激发后以及在皮肤同种异体移植后，分别从组织学和亚微观水平分析了引流淋巴结中的体液免疫反应。将这些具有“孤立B细胞系统”的动物的时间序列研究与正常未照射兔子的类似实验进行了比较。在淋巴结中确定了非胸腺依赖性和胸腺依赖性浆细胞反应的起始部位。描述并讨论了抗体形成（-B-）细胞前体即边缘区细胞的（超）结构特征。展示了两条在（亚）微观上可识别的浆细胞系分化的后代。
