Veldman J E, Keuning F J
Virchows Arch B Cell Pathol. 1978 Oct 16;28(3):203-16. doi: 10.1007/BF02889070.
Three times sublethal total body X-irradiation with thymus shielding--at 2 weeks' intervals--delineated a temporarily B-cell deprived animal model, only reconstituted with recently thymus-derived cells. The thymusdependent areas of peripheral lymphoid tissue-repleted with T-cells--are described. The cellular immune capacity of these animals with an "isolated T-cell system" was analyzed by means of skin allografting. Histological and autoradiographic studies were performed in draining lymph nodes after a variety of antigenic stimuli: skin allografts, S. java vaccin, horse-gamma-globulin, horse spleen ferritin and a contact sensitizer (Oxazolone).
对动物进行三次亚致死剂量的全身X射线照射，并对胸腺进行屏蔽，照射间隔为两周，由此构建了一个暂时缺乏B细胞的动物模型，该模型仅由最近来源于胸腺的细胞进行重建。文中描述了外周淋巴组织中富含T细胞的胸腺依赖区。通过皮肤同种异体移植分析了这些具有“孤立T细胞系统”的动物的细胞免疫能力。在给予多种抗原刺激后，对引流淋巴结进行了组织学和放射自显影研究，这些抗原刺激包括皮肤同种异体移植、爪哇沙门氏菌疫苗、马γ球蛋白、马脾铁蛋白和一种接触性致敏剂（恶唑酮）。
