Newkirk M M, Watanabe Duffy K N, Leclerc J, Lambert N, Shiroky J B
Department of Medicine, Montreal General Hospital, McGill University, Canada.
Br J Rheumatol. 1994 Apr;33(4):317-22. doi: 10.1093/rheumatology/33.4.317.
The frequency of latent viral infection by cytomegalovirus (CMV), Epstein-Barr virus (EBV) and herpes virus-6 (HHV-6) was investigated in patients with RA with or without Sjögren's syndrome (SS) and in normal controls. Virus presence was determined by polymerase chain amplification of DNA isolated from peripheral blood mononuclear or polymorphonuclear cells and/or saliva-derived mononuclear/epithelial cells. Anti-viral antibodies and autoantibodies were also assayed. Patients with RA both with and without SS were found to have a significantly increased frequency of latent viral infection (two-fold higher, P = 0.035 for EBV and seven-fold higher, P = 0.018 for HHV-6) compared to normal controls but only in cells isolated from saliva. The increased frequency of virally infected cells from the saliva of patients with RA, regardless of the SS status, when compared to normal controls may reflect the ongoing inflammatory process, the impact of therapy and/or a less effective local immune responsiveness.
在患有或未患有干燥综合征（SS）的类风湿关节炎（RA）患者以及正常对照中，研究了巨细胞病毒（CMV）、EB病毒（EBV）和疱疹病毒6型（HHV - 6）潜伏病毒感染的频率。通过聚合酶链反应扩增从外周血单核或多形核细胞和/或唾液来源的单核/上皮细胞中分离的DNA来确定病毒的存在。还检测了抗病毒抗体和自身抗体。与正常对照相比，发现患有和未患有SS的RA患者潜伏病毒感染频率显著增加（EBV高两倍，P = 0.035；HHV - 6高七倍，P = 0.018），但仅在从唾液中分离的细胞中。与正常对照相比，无论SS状态如何，RA患者唾液中病毒感染细胞频率的增加可能反映了正在进行的炎症过程、治疗的影响和/或局部免疫反应性较低。
