Saito I, Nishimura S, Kudo I, Fox R I, Moro I
Department of Pathology, School of Dentistry, Nihon University, Tokyo, Japan.
Arch Oral Biol. 1991;36(11):779-84. doi: 10.1016/0003-9969(91)90026-q.
Direct detection of these viruses was made by using the PCR for amplifying viral DNA. In virtually all adults low levels of the herpesvirus can be detected. Therefore it is necessary to quantitate the amount of viral DNA, and a method to compare the plasmid containing the cloned target gene was used here. After 35 cycles of amplification, 10 copies of the viral DNA per 100 microliters saliva were detected. The PCR was used to detect increased levels of EBV and HHV-6 in saliva from patients with lymphoproliferative diseases, suggesting that these viruses may play a part in their pathogenesis. Viral detection by such highly sensitive methods as PCR may allow better monitoring of medication, as well as early detection of EBV- and HHV-6 related diseases that may arise in these patients. The great sensitivity of PCR and its ability to analyse very small samples make this technique most suitable for clinical diagnosis.
通过使用聚合酶链反应（PCR）扩增病毒DNA来直接检测这些病毒。实际上，在所有成年人中都能检测到低水平的疱疹病毒。因此，有必要对病毒DNA的量进行定量，这里使用了一种比较含有克隆靶基因的质粒的方法。经过35个循环的扩增后，在每100微升唾液中检测到10个病毒DNA拷贝。PCR被用于检测淋巴增殖性疾病患者唾液中EB病毒（EBV）和人疱疹病毒6型（HHV-6）水平的升高，这表明这些病毒可能在其发病机制中起作用。通过像PCR这样的高灵敏度方法进行病毒检测，可能有助于更好地监测药物治疗，以及早期发现这些患者可能出现的与EBV和HHV-6相关的疾病。PCR的高灵敏度及其分析非常小样本的能力使其非常适合临床诊断。
