Preece N E, Jackson G D, Houseman J A, Duncan J S, Williams S R
Department of Biophysics, Hunterian Institute, London, England.
Epilepsia. 1994 Mar-Apr;35(2):431-6. doi: 10.1111/j.1528-1157.1994.tb02456.x.
1H Nuclear magnetic resonance ([1H]NMR) spectroscopy was used to detect elevation of gamma-aminobutyric acid (GABA) in rat brain after administration of the antiepileptic drug vigabatrin (VGB). Rats were treated for 3 weeks with VGB added to their drinking water to deliver a dose of 250 mg/kg body weight per day. NMR spectroscopy was performed noninvasively in vivo, and a GABA concentration of 6.0 +/- 2.3 mmol/kg wet weight (mean +/- SD, n = 5) was measured. GABA could not be detected in control animals in vivo. Postmortem GABA levels of 1.3 +/- 0.5 and 4.5 +/- 1.0 mmol/kg (mean +/- SD, n = 5) were measured in perchloric acid extracts of frozen brain from control and treated animals, respectively. Noninvasive measurement of increased cerebral GABA should allow detailed studies of the pharmacology of GABA-increasing drugs in vivo. With future developments, these measurements may be feasible in human subjects.
使用氢核磁共振波谱（[1H]NMR）来检测抗癫痫药物氨己烯酸（VGB）给药后大鼠脑内γ-氨基丁酸（GABA）的升高情况。将VGB添加到大鼠饮用水中，以每天250mg/kg体重的剂量对大鼠进行为期3周的治疗。在体内以非侵入性方式进行核磁共振波谱分析，测得GABA浓度为6.0±2.3mmol/kg湿重（平均值±标准差，n = 5）。在对照动物体内未检测到GABA。分别在对照动物和治疗动物的冷冻脑的高氯酸提取物中测得死后GABA水平为1.3±0.5和4.5±1.0mmol/kg（平均值±标准差，n = 5）。对脑内GABA增加进行非侵入性测量应有助于对体内增加GABA药物的药理学进行详细研究。随着未来的发展，这些测量在人类受试者中可能是可行的。
