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GDI1编码一种GDP解离抑制剂，它在酵母分泌途径中起关键作用。

GDI1 encodes a GDP dissociation inhibitor that plays an essential role in the yeast secretory pathway.

作者信息

Garrett M D, Zahner J E, Cheney C M, Novick P J

机构信息

Department of Cell Biology, Yale University School of Medicine, New Haven, CT 06510.

出版信息

EMBO J. 1994 Apr 1;13(7):1718-28. doi: 10.1002/j.1460-2075.1994.tb06436.x.

DOI:10.1002/j.1460-2075.1994.tb06436.x
PMID:8157010
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC395005/
Abstract

GTP binding proteins of the Sec4/Ypt/rab family regulate distinct vesicular traffic events in eukaryotic cells. We have cloned GDI1, an essential homolog of bovine rab GDI (GDP dissociation inhibitor) from the yeast Saccharomyces cerevisiae. Analogous to the bovine protein, purified Gdi1p slows the dissociation of GDP from Sec4p and releases the GDP-bound form from yeast membranes. Depletion of Gdi1p in vivo leads to loss of the soluble pool of Sec4p and inhibition of protein transport at multiple stages of the secretory pathway. Complementation analysis indicates that GDI1 is allelic to sec19-1. These results establish that Gdi1p plays an essential function in membrane traffic and are consistent with a role for Gdi1p in the recycling of proteins of the Sec4/Ypt/rab family from their target membranes back to their vesicular pools.

摘要

Sec4/Ypt/rab家族的GTP结合蛋白调节真核细胞中不同的囊泡运输事件。我们从酿酒酵母中克隆了GDI1，它是牛rab GDI（GDP解离抑制剂）的一个必需同源物。与牛蛋白类似，纯化的Gdi1p减缓了GDP从Sec4p上的解离，并从酵母膜上释放出结合GDP的形式。体内Gdi1p的缺失导致Sec4p可溶性池的丧失，并在分泌途径的多个阶段抑制蛋白质运输。互补分析表明GDI1与sec19-1等位。这些结果表明Gdi1p在膜运输中起重要作用，并且与Gdi1p在将Sec4/Ypt/rab家族的蛋白质从其靶膜循环回到其囊泡池中的作用一致。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/81c4/395005/a1cde8435b94/emboj00055-0238-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/81c4/395005/4c18b8df8c06/emboj00055-0233-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/81c4/395005/bd67059943ae/emboj00055-0234-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/81c4/395005/9d376086772c/emboj00055-0235-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/81c4/395005/0619c75f4560/emboj00055-0236-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/81c4/395005/69db273a3916/emboj00055-0236-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/81c4/395005/a754cdc9b00e/emboj00055-0237-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/81c4/395005/f71b47505c6d/emboj00055-0238-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/81c4/395005/a1cde8435b94/emboj00055-0238-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/81c4/395005/4c18b8df8c06/emboj00055-0233-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/81c4/395005/bd67059943ae/emboj00055-0234-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/81c4/395005/9d376086772c/emboj00055-0235-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/81c4/395005/0619c75f4560/emboj00055-0236-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/81c4/395005/69db273a3916/emboj00055-0236-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/81c4/395005/a754cdc9b00e/emboj00055-0237-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/81c4/395005/f71b47505c6d/emboj00055-0238-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/81c4/395005/a1cde8435b94/emboj00055-0238-b.jpg

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