Hollingsworth M A, Strawhecker J M, Caffrey T C, Mack D R
Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, Omaha.
Int J Cancer. 1994 Apr 15;57(2):198-203. doi: 10.1002/ijc.2910570212.
We examined the steady-state expression levels of mRNA for the MUC1, MUC2, MUC3 and MUC4 gene products in 12 pancreatic tumor cell lines, 6 colon tumor cell lines, and one ileocecal tumor cell line. The results showed that 10 of 12 pancreatic tumor cell lines expressed MUC1 mRNA and that 7 of these 12 lines also expressed relatively high levels of MUC4 mRNA. In contrast, MUC2 mRNA was expressed at only low levels and MUC3 was not detected in the pancreatic tumor cell lines. All 7 intestinal tumor cell lines examined expressed MUC2, and 5 of 7 expressed MUC3; however only one expressed significant levels of MUC1 and 2 expressed low levels of MUC4 mRNA. This report of high levels of MUC4 mRNA expression by pancreatic tumor cells raises the possibility that mucin carbohydrate epitopes defined by antibodies such as DuPan 2 may be expressed on a second mucin core protein produced by pancreatic tumor cells.
我们检测了12种胰腺肿瘤细胞系、6种结肠肿瘤细胞系和1种回盲部肿瘤细胞系中MUC1、MUC2、MUC3和MUC4基因产物的mRNA稳态表达水平。结果显示，12种胰腺肿瘤细胞系中有10种表达MUC1 mRNA，其中12种细胞系中的7种还表达相对高水平的MUC4 mRNA。相比之下，MUC2 mRNA仅低水平表达，胰腺肿瘤细胞系中未检测到MUC3。检测的所有7种肠道肿瘤细胞系均表达MUC2，7种中有5种表达MUC3；然而，只有1种表达显著水平的MUC1，2种表达低水平的MUC4 mRNA。胰腺肿瘤细胞中MUC4 mRNA高水平表达的这一报告增加了由诸如DuPan 2等抗体定义的粘蛋白碳水化合物表位可能在胰腺肿瘤细胞产生的第二种粘蛋白核心蛋白上表达的可能性。
