Balagué C, Gambús G, Carrato C, Porchet N, Aubert J P, Kim Y S, Real F X
Departament d'Immunologia, Universitat Autònoma de Barcelona, Spain.
Gastroenterology. 1994 Apr;106(4):1054-61. doi: 10.1016/0016-5085(94)90767-6.
BACKGROUND/AIMS: Neoplastic transformation of epithelial cells is commonly associated with altered synthesis and structure of mucin glycoproteins. The aim of the study was to determine if altered mucin gene expression takes place in pancreas cancer.
To examine mucin gene expression in normal pancreas and pancreas cancer, antibodies detecting the MUC1, MUC2, MUC5B, and MUC5C apomucins were used in immunohistochemical techniques and complementary DNA probes specific for the MUC1-MUC5 genes were used in Northern blots.
MUC1 is the major apomucin expressed in normal pancreas, whereas MUC2-MUC5 are weakly expressed or undetectable. In pancreas cancer tissues and cell lines, increased expression of MUC2, MUC4, and MUC5C is shown. The cytoplasmic expression of MUC2 and MUC5C in tumor cells suggests that these apomucins are underglycosylated and abnormally compartmentalized.
Enhanced expression of MUC2, MUC4, and MUC5C genes is a frequent event in pancreas cancer and may contribute to the alterations in the biochemical structure of pancreas cancer mucins.
背景/目的：上皮细胞的肿瘤转化通常与粘蛋白糖蛋白的合成和结构改变有关。本研究的目的是确定胰腺癌中是否发生粘蛋白基因表达改变。
为检测正常胰腺和胰腺癌中的粘蛋白基因表达，在免疫组织化学技术中使用检测MUC1、MUC2、MUC5B和MUC5C脱辅基粘蛋白的抗体，并在Northern印迹中使用针对MUC1 - MUC5基因的互补DNA探针。
MUC1是正常胰腺中表达的主要脱辅基粘蛋白，而MUC2 - MUC5表达较弱或无法检测到。在胰腺癌组织和细胞系中，显示MUC2、MUC4和MUC5C表达增加。肿瘤细胞中MUC2和MUC5C的细胞质表达表明这些脱辅基粘蛋白糖基化不足且定位异常。
MUC2、MUC4和MUC5C基因的表达增强在胰腺癌中很常见，可能导致胰腺癌粘蛋白生化结构的改变。
