Dykes G A, Britz T J, von Holy A
Department of Microbiology, University of the Witwatersrand, Johannesburg, South Africa.
J Appl Bacteriol. 1994 Mar;76(3):246-52. doi: 10.1111/j.1365-2672.1994.tb01623.x.
Sixty-one lactic acid bacteria from spoiled vacuum-packaged vienna sausages and 15 reference strains were tested for 72 phenotypic characteristics. An identification key and a computer data base, both specific for lactic acid bacteria from meat sources, were used for identification and the results were compared. There was a high correlation (86.9%) between the two procedures in the identification of strains to genus level. However, only a 54.8% correlation was obtained in identifying strains to the species level. With numerical taxonomy (Ssm matching coefficient with average linkage clustering) 60 strains were recovered in six clusters at the 89% similarity level. While most Leuconostoc strains clustered separately from the Lactobacillus strains, the identity of many leuconostocs was not clarified. The presence of a heterogeneous cluster containing typical and 'atypical' strains of the Lactobacillus saké/curvatus group and a separate homogeneous Lact. curvatus cluster was noted. Closer examination of the data suggested that the 'atypical' lactobacilli were all strains of Lact. saké.
对来自变质真空包装维也纳香肠的61株乳酸菌和15株参考菌株进行了72种表型特征测试。使用了一个专门针对肉类来源乳酸菌的鉴定检索表和一个计算机数据库进行鉴定，并对结果进行了比较。在菌株鉴定到属水平时，两种方法之间存在高度相关性（86.9%）。然而，在将菌株鉴定到种水平时，仅获得了54.8%的相关性。采用数值分类法（Ssm匹配系数与平均连锁聚类），在89%的相似性水平下，60株菌株被归为6个聚类。虽然大多数明串珠菌菌株与乳酸杆菌菌株分开聚类，但许多明串珠菌的身份并未明确。注意到存在一个包含清酒乳杆菌/弯曲乳杆菌组典型和“非典型”菌株的异质聚类以及一个单独的均质弯曲乳杆菌聚类。对数据的进一步检查表明，“非典型”乳酸杆菌均为清酒乳杆菌菌株。
