Dykes G, von Holy A
Department of Microbiology, University of the Witwatersrand, Johannesburg, South Africa.
J Basic Microbiol. 1993;33(3):169-77. doi: 10.1002/jobm.3620330306.
To resolve the taxonomic status of sixty-one lactic acid bacteria isolated from spoiled, vacuum packaged vienna sausages, a computer assisted analysis of total soluble protein profiles was undertaken. Sixty-two% of the strains in the study were recovered in fourteen clusters at the 72% similarity level, indicating high degree of heterogeneity among the strains examined. Two clusters representing Leuconostoc species were the largest and most homogeneous, containing twenty strains between them. One cluster contained both typical Lactobacillus saké and Lactobacillus curvatus strains, indicating the inability of protein profiles to consistently differentiate between all strains of these two species. The presence of an independent Lactobacillus curvatus grouping and the clustering of some atypical strains with Lactobacillus saké isolates, however, indicated that the atypical strains may be closely related to Lactobacillus saké.
为了确定从变质真空包装维也纳香肠中分离出的61株乳酸菌的分类地位，我们对总可溶性蛋白谱进行了计算机辅助分析。研究中62%的菌株在72%的相似性水平上被归为14个簇，这表明所检测的菌株之间存在高度异质性。代表明串珠菌属的两个簇最大且最均匀，它们之间共包含20个菌株。一个簇同时包含典型的清酒乳杆菌和弯曲乳杆菌菌株，这表明蛋白谱无法始终区分这两个物种的所有菌株。然而，独立的弯曲乳杆菌分组的存在以及一些非典型菌株与清酒乳杆菌分离株的聚类表明，这些非典型菌株可能与清酒乳杆菌密切相关。
