Miron J, Duncan S H, Stewart C S
Metabolic Unit, Institute of Animal Science, ARO, Volcani Center, Bet Dagan, Israel.
J Appl Bacteriol. 1994 Mar;76(3):282-7. doi: 10.1111/j.1365-2672.1994.tb01629.x.
Pure cultures and pair-combinations of strains representative of the rumen cellulolytic species Ruminococcus flavefaciens, Fibrobacter succinogenes and Butyrivibrio fibrisolvens were grown on cell-wall materials from barley straw. Of the pure cultures, R. flavefaciens solubilized straw most rapidly. The presence of B. fibrisolvens, which was unable to degrade straw extensively in pure culture, increased the solubilization of dry matter by R. flavefaciens and the solubilization of cell-wall carbohydrates by both R. flavefaciens and F. succinogenes. During fermentation, both R. flavefaciens and F. succinogenes released bound glucose and free and bound arabinose and xylose into solution. The accumulation of these sugars, especially arabinose and xylose, was greatly reduced in co-cultures containing B. fibrisolvens, suggesting that significant interspecies cross feeding of the products of hemicellulose hydrolysis (particularly soluble bound xylose released by F. succinogenes) occurs during straw degradation by mixed cultures containing this species.
瘤胃纤维素分解菌黄化瘤胃球菌（Ruminococcus flavefaciens）、产琥珀酸纤维杆菌（Fibrobacter succinogenes）和溶纤维丁酸弧菌（Butyrivibrio fibrisolvens）的纯培养物以及菌株组合，在大麦秸秆细胞壁材料上生长。在纯培养物中，黄化瘤胃球菌溶解秸秆的速度最快。在纯培养中不能大量降解秸秆的溶纤维丁酸弧菌的存在，增加了黄化瘤胃球菌对干物质的溶解以及黄化瘤胃球菌和产琥珀酸纤维杆菌对细胞壁碳水化合物的溶解。在发酵过程中，黄化瘤胃球菌和产琥珀酸纤维杆菌都将结合态葡萄糖、游离态和结合态阿拉伯糖及木糖释放到溶液中。在含有溶纤维丁酸弧菌的共培养物中，这些糖尤其是阿拉伯糖和木糖的积累量大幅减少，这表明在含有该菌的混合培养物降解秸秆过程中，半纤维素水解产物（特别是产琥珀酸纤维杆菌释放的可溶性结合态木糖）存在显著的种间交叉利用。
