Saluzzi L, Smith A, Stewart C S
Rowett Research Institute, Bucksburn, Aberdeen, UK.
J Gen Microbiol. 1993 Nov;139(11):2865-73. doi: 10.1099/00221287-139-11-2865.
Marker components of the phospholipids of Ruminococcus flavefaciens and Fibrobacter succinogenes were identified for studies on the degradation of forage by these bacteria growing in mixed culture. The principal fatty acid methyl esters and dimethyl acetals detected varied between strains and were influenced by the addition of a mixture of higher volatile fatty acids and vitamins to the medium, but these effects were small compared to the differences between the species. When two strains of R. flavefaciens were grown on a mixture of clover and ryegrass, and on barley straw in the presence or absence of two strains of F. succinogenes, the solubilization of plant material tended to be lowered by the presence of F. succinogenes. R. flavefaciens was the predominant bacterium among colonies recovered from roll tubes, and the phospholipids were primarily those of R. flavefaciens. Analysis of the culture supernatant liquids showed that F. succinogenes produced greater amounts of free and bound xylose from both clover and straw than did R. flavefaciens. With both forages, cultures containing the two species produced more soluble free arabinose, and less soluble-bound arabinose, than either species grown alone.
为了研究在混合培养中生长的这些细菌对草料的降解作用，对黄化瘤胃球菌和琥珀酸纤维杆菌的磷脂标记成分进行了鉴定。检测到的主要脂肪酸甲酯和二甲基缩醛在不同菌株之间存在差异，并受到向培养基中添加高挥发性脂肪酸和维生素混合物的影响，但与物种之间的差异相比，这些影响较小。当两株黄化瘤胃球菌在三叶草和黑麦草的混合物上生长，以及在有或没有两株琥珀酸纤维杆菌存在的情况下在大麦秸秆上生长时，琥珀酸纤维杆菌的存在往往会降低植物材料的溶解率。黄化瘤胃球菌是从滚动管中回收的菌落中的优势细菌，磷脂主要是黄化瘤胃球菌的磷脂。对培养上清液的分析表明，琥珀酸纤维杆菌从三叶草和秸秆中产生的游离木糖和结合木糖比黄化瘤胃球菌更多。对于这两种草料，与单独培养的任何一种相比，含有这两种菌的培养物产生的可溶性游离阿拉伯糖更多，而可溶性结合阿拉伯糖更少。
