Elorza M V, Sentandreu R, Ruiz-Herrera J
Sección de Microbiología, Facultat de Farmacia, Universitat de Valencia, Spain.
J Bacteriol. 1994 Apr;176(8):2318-25. doi: 10.1128/jb.176.8.2318-2325.1994.
A method was devised for the isolation of yeast monomorphic (LEV) mutants of Candida albicans. By this procedure, about 20 stable yeast-like mutants were isolated after mutagenesis with ethyl methane sulfonate. The growth rate of the mutants in different carbon sources, both fermentable and not, was indistinguishable from that of the parental strain, but they were unable to grow as mycelial forms after application of any of the common effective inducers, i.e., heat shock, pH alterations, proline addition, or use of GlcNAc as the carbon source. Studies performed with one selected strain demonstrated that it had severe alterations in the chemical composition of the cell wall, mainly in the levels of chitin and glucans, and in specific mannoproteins, some of them recognizable by specific polyclonal and monoclonal antibodies. It is suggested that these structural alterations hinder the construction of a normal hyphal wall.
设计了一种用于分离白色念珠菌酵母单态性（LEV）突变体的方法。通过该程序，在用甲磺酸乙酯诱变后分离出约20个稳定的酵母样突变体。这些突变体在不同碳源（包括可发酵和不可发酵的碳源）中的生长速率与亲代菌株无异，但在应用任何常见的有效诱导剂后，即热休克、pH改变、添加脯氨酸或使用GlcNAc作为碳源后，它们无法以菌丝形式生长。对一个选定菌株进行的研究表明，其细胞壁的化学成分发生了严重改变，主要是几丁质和葡聚糖的水平，以及特定的甘露糖蛋白，其中一些可被特定的多克隆和单克隆抗体识别。有人认为，这些结构改变阻碍了正常菌丝壁的构建。
