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利用伴刀豆球蛋白A-铁蛋白对甘露糖蛋白进行标记,对白色念珠菌酵母和菌丝生长过程中的细胞壁扩张进行电子显微镜研究。

An electron microscopy study of wall expansion during Candida albicans yeast and mycelial growth using concanavalin A-ferritin labelling of mannoproteins.

作者信息

Rico H, Herrero E, Miragall F, Sentandreu R

机构信息

Departament de Microbiología, Facultat de Farmàcia, Universidat de València, Spain.

出版信息

Arch Microbiol. 1991;156(2):111-4. doi: 10.1007/BF00290982.

DOI:10.1007/BF00290982
PMID:1781727
Abstract

Depending upon growth temperature, Candida albicans can exhibit two different morphologies, a budding yeast or a mycelium. By studying the distribution of concanavalin A-ferritin particles on the cell wall surface during bud and germ tube formation, we have elucidated the way cell wall extension occurs. Both processes initially require the localized lysis of the wall in order to allow the incorporation of the newly synthesized material. Later on, the cell wall behaves as an elastic structure, allowing extension by an intussusception process and, as a consequence, cell growth.

摘要

根据生长温度,白色念珠菌可呈现两种不同的形态,即出芽酵母或菌丝体。通过研究伴刀豆球蛋白A-铁蛋白颗粒在芽和芽管形成过程中在细胞壁表面的分布,我们阐明了细胞壁延伸的方式。这两个过程最初都需要细胞壁的局部裂解,以便纳入新合成的物质。随后,细胞壁表现为一种弹性结构,通过套入生长过程实现延伸,从而实现细胞生长。

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本文引用的文献

1
Cytochemical and ultrastructural studies of Candida albicans. II. Evidence for a cell wall coat using concanavalin A.白色念珠菌的细胞化学与超微结构研究。II. 使用伴刀豆球蛋白A证明细胞壁被膜的存在
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用一种特异性针对 Als1 的单克隆抗体显示白念珠菌细胞表面抗原的不均匀分布。
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A characterization of pH-regulated dimorphism in Candida albicans.白色念珠菌中pH调节二态性的特征描述。
Mycopathologia. 1984 Mar 15;85(1-2):21-30. doi: 10.1007/BF00436698.
6
Calcofluor white alters the assembly of chitin fibrils in Saccharomyces cerevisiae and Candida albicans cells.荧光增白剂会改变酿酒酵母和白色念珠菌细胞中几丁质原纤维的组装。
J Gen Microbiol. 1983 May;129(5):1577-82. doi: 10.1099/00221287-129-5-1577.
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Filament ring formation in the dimorphic yeast Candida albicans.二态性酵母白色念珠菌中的丝状环形成。
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A low-viscosity epoxy resin embedding medium for electron microscopy.一种用于电子显微镜的低粘度环氧树脂包埋介质。
J Ultrastruct Res. 1969 Jan;26(1):31-43. doi: 10.1016/s0022-5320(69)90033-1.
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Cell wall formation in yeast. An electron microscopic autoradiographic study.酵母细胞壁的形成。一项电子显微镜放射自显影研究。
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Autoradiographic study of mannan incorporation into the growing cell walls of Saccharomyces cerevisiae.甘露聚糖掺入酿酒酵母生长细胞壁的放射自显影研究。
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