Quistad G B, Skinner W S
Sandoz Agro, Inc., Palo Alto, California 94304.
J Biol Chem. 1994 Apr 15;269(15):11098-101.
About 50 peptide toxins were purified from venom of the primitive hunting spider, Plectreurys tristis. Bioassay by injection into larval Heliothis virescens (tobacco budworm) allowed selection of nine toxins for analysis of amino acid sequences. Total sequences were determined for six of the more insecticidal peptides (46-49 amino acids) and three contained free carboxyl-terminal amino acids. These toxins (plectoxins) are paralytic and/or lethal when injected into insect pests such as the lepidopterous larvae H. virescens, Spodoptera exigua (beet armyworm), and Manduca sexta (tobacco hornworm). We expect these plectoxins to be useful in insect control after delivery by a suitable agent such as a recombinant baculovirus.
从原始的狩猎蜘蛛——三突花蛛的毒液中纯化出了约50种肽毒素。通过注射到烟草天蛾幼虫体内进行生物测定，从而筛选出9种毒素用于氨基酸序列分析。测定了6种杀虫性更强的肽（46 - 49个氨基酸）的完整序列，另外3种含有游离的羧基末端氨基酸。当将这些毒素（花蛛毒素）注射到害虫体内时，如鳞翅目幼虫烟草天蛾、甜菜夜蛾和烟草天蛾，它们具有麻痹和/或致死作用。我们期望这些花蛛毒素在通过合适的载体（如重组杆状病毒）递送后，能用于害虫防治。
