Taylor K, Copley C G
ICI Central Toxicology Laboratory Biotechnology Department, Macclesfield, Cheshire, UK.
Lab Anim. 1994 Jan;28(1):26-30. doi: 10.1258/002367794781065807.
The exclusive use of serology, namely immunofluorescent antibody (IFA), for the diagnosis of rodent parvovirus infection is limited to detecting IgG antibodies which develop some time after the appearance of clinical symptoms, whilst conventional viral isolation, using tissue culture, is both expensive and time consuming. Polymerase chain reaction (PCR) as a diagnostic method has the potential to overcome these problems. However, it requires detailed knowledge of the genetic code of the target organism and careful selection of the primers used. This paper describes preliminary findings in a PCR assay which detected Kilham rat virus, minute virus of mice and Toolan's H1 virus.
仅使用血清学方法，即免疫荧光抗体法（IFA）诊断啮齿动物细小病毒感染，仅限于检测临床症状出现一段时间后产生的IgG抗体，而使用组织培养进行传统病毒分离既昂贵又耗时。聚合酶链反应（PCR）作为一种诊断方法有潜力克服这些问题。然而，它需要对目标生物体的遗传密码有详细了解，并仔细选择所用的引物。本文描述了一种PCR检测方法的初步结果，该方法检测了基尔汉姆大鼠病毒、小鼠微小病毒和图兰H1病毒。
