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1
Identification and propagation of a putative immunosuppressive orphan parvovirus in cloned T cells.在克隆的T细胞中鉴定和增殖一种假定的免疫抑制性细小病毒。
J Immunol. 1993 Jan 15;150(2):419-28.
2
In vivo studies with an "orphan" parvovirus of mice.对小鼠一种“孤儿”细小病毒的体内研究。
Lab Anim Sci. 1993 Apr;43(2):175-82.
3
Contamination of transplantable tumors, cell lines, and monoclonal antibodies with rodent viruses.可移植肿瘤、细胞系和单克隆抗体被啮齿动物病毒污染。
Lab Anim Sci. 1993 Aug;43(4):296-300.
4
Molecular characterization of a newly recognized mouse parvovirus.一种新发现的小鼠细小病毒的分子特征
J Virol. 1994 Oct;68(10):6476-86. doi: 10.1128/JVI.68.10.6476-6486.1994.
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Effects of Kilham rat virus on natural killer cell-mediated cytotoxicity in brown Norway and Wistar Furth rats.基尔汉姆大鼠病毒对棕色挪威大鼠和威斯塔·弗思大鼠自然杀伤细胞介导的细胞毒性的影响。
Am J Vet Res. 1984 Jan;45(1):200-2.
6
Enzyme-linked immunosorbent assay in the diagnosis of Kilham rat virus infection in rats.酶联免疫吸附测定法在大鼠基尔汉姆大鼠病毒感染诊断中的应用
Lab Anim. 1984 Oct;18(4):364-70. doi: 10.1258/002367784780865289.
7
Spontaneous hepatitis and cerebellar "hypoplasia" in suckling rats due to congenital infections with rat virus.由于感染大鼠病毒导致新生大鼠出现自发性肝炎和小脑“发育不全”
Am J Pathol. 1966 Sep;49(3):457-75.
8
Parvoiruses as contaminants of permanent human cell lines. I. Virus isolation from 1960-1970.作为人类永久细胞系污染物的Parvoiruses。I. 1960 - 1970年病毒分离情况
Arch Gesamte Virusforsch. 1971;35(1):80-90. doi: 10.1007/BF01249755.
9
Murine virus contaminants of leukemia viruses and transplantable tumors.白血病病毒和可移植肿瘤的鼠类病毒污染物
J Natl Cancer Inst. 1972 Oct;49(4):1139-43.
10
Minute virus of mice. II. Prevalence, epidemiology, and occurrence as a contaminant of transplanted tumors.小鼠微小病毒。II. 作为移植瘤污染物的流行情况、流行病学及发生情况。
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杆状病毒表达重组细小病毒NS1蛋白及其在啮齿动物血清学检测中的应用。

Expression of recombinant parvovirus NS1 protein by a baculovirus and application to serologic testing of rodents.

作者信息

Riley L K, Knowles R, Purdy G, Salomé N, Pintel D, Hook R R, Franklin C L, Besch-Williford C L

机构信息

Department of Veterinary Pathobiology, University of Missouri, Columbia 65211, USA.

出版信息

J Clin Microbiol. 1996 Feb;34(2):440-4. doi: 10.1128/jcm.34.2.440-444.1996.

DOI:10.1128/jcm.34.2.440-444.1996
PMID:8789034
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC228816/
Abstract

A recombinant baculovirus containing the NS1 gene of minute virus of mice was constructed. Optimal expression of the recombinant NS1 protein (rNS1) was achieved by infecting Trichoplusa ni High Five cells at a multiplicity of 10 and incubating them for 72 h postinfection. An enzyme-linked immunosorbent assay (ELISA) with rNS1 as the antigen was evaluated for serologic testing of laboratory rodents. The rNS1 ELISA proved to be a more sensitive method for the detection of antibodies to recently recognized rodent parvovirus species (mouse orphan parvovirus and rat orphan parvovirus) and prototypic parvovirus species (minute virus of mice, Kilham's rat virus, and H-1) than were conventional parvovirus ELISAs that use whole parvovirus virions.

摘要

构建了一种含有小鼠微小病毒NS1基因的重组杆状病毒。通过以感染复数10感染草地贪夜蛾High Five细胞并在感染后孵育72小时，实现了重组NS1蛋白（rNS1）的最佳表达。评估了以rNS1作为抗原的酶联免疫吸附测定（ELISA）用于实验啮齿动物的血清学检测。与使用全细小病毒病毒粒子的传统细小病毒ELISA相比，rNS1 ELISA被证明是一种更灵敏的方法，用于检测针对最近识别的啮齿动物细小病毒种类（小鼠孤儿细小病毒和大鼠孤儿细小病毒）和原型细小病毒种类（小鼠微小病毒、基尔汉姆大鼠病毒和H-1）的抗体。