Sayama K, Kaneko T, Matsuzawa A
Laboratory of General Biology, Meijigakuin University, Yokohama, Japan.
Lab Anim. 1994 Jan;28(1):44-9. doi: 10.1258/002367794781065889.
Mammary tumours developed in 110 (95.7%) of 115 DDD/1-Mtv-2/Mtv-2 (DDD/1-Mtv-2) and 24 (47.1%) of 51 DDD/1fDDD/1-Mtv-2 (DDD/1fMtv-2) force-bred female mice during a one-year period. The mean tumour age +/- SE was 220 +/- 7 and 269 +/- 7 days, respectively. These tumours were examined for responsiveness to pregnancies by comparing their growth after transplantation between virgin and breeding recipients. Of 73 tumours from DDD/1-Mtv-2 mice, 9 (12%) were completely pregnancy-dependent (CPD), 3 (4%) pregnancy-dependent (PD), 9 (12%) pregnancy-responsive (PR), and 52 (71%) pregnancy-independent (PI), and of 25 tumours from DDD/1fMtv-2 mice, one (4%) was CPD, one (4%) PR, and 23 (92%) PI. Although most tumours were heterogeneous in morphology and there was no clear relation between morphology and PD properties, most CPD tumours were type P and considered to be connected with mammary plaques. When 9 CPD tumours from DDD/1-Mtv-2 mice were serially transplanted in breeders, 6, 2 and one progressed to lose pregnancy dependence within 3, 8 and 18 generations, respectively. DDD/1-Mtv-2 mice will provide a model for studies on progression of mammary tumours from hormone-dependent to autonomous states.
在为期一年的时间里，115只DDD/1-Mtv-2/Mtv-2（DDD/1-Mtv-2）强制配种雌性小鼠中有110只（95.7%）发生了乳腺肿瘤，51只DDD/1fDDD/1-Mtv-2（DDD/1fMtv-2）强制配种雌性小鼠中有24只（47.1%）发生了乳腺肿瘤。肿瘤出现的平均年龄±标准误分别为220±7天和269±7天。通过比较在未生育和生育受体中移植后的生长情况，检测这些肿瘤对妊娠的反应性。在来自DDD/1-Mtv-2小鼠的73个肿瘤中，9个（12%）完全依赖妊娠（CPD），3个（4%）依赖妊娠（PD），9个（12%）对妊娠有反应（PR），52个（71%）不依赖妊娠（PI）；在来自DDD/1fMtv-2小鼠的25个肿瘤中，1个（4%）为CPD，1个（4%）为PR，23个（92%）为PI。尽管大多数肿瘤在形态上是异质性的，且形态与PD特性之间没有明确的关系，但大多数CPD肿瘤为P型，被认为与乳腺斑块有关。当将来自DDD/1-Mtv-2小鼠的9个CPD肿瘤连续移植到生育小鼠中时，分别在第3、8和18代中有6个、2个和1个逐渐失去对妊娠的依赖性。DDD/1-Mtv-2小鼠将为研究乳腺肿瘤从激素依赖性向自主状态的进展提供一个模型。
