Wang Y, Selden A C, Morgan N, Stamp G W, Hodgson H J
Department of Medicine, Royal Postgraduate Medical School, London, United Kingdom.
Am J Pathol. 1994 Apr;144(4):675-82.
This study reports expression of mRNA for the growth modulator hepatocyte growth factor/scatter factor (HGF/SF) in both benign and malignant human mammary epithelium by in situ hybridization. In benign breast tissue expression was prominent in areas of adenosis and in peripheral acinic cells in lactating breast; in malignant epithelium expression was seen in 15 of 21 cases of in situ and invasive breast cancer. In some cases of invasive ductal carcinoma stronger labeling appeared to be associated with areas of tubule formation compared with areas of infiltrating growth, although this was not a universal finding. In contrast, two examples each of in situ comedo carcinoma and invasive lobular carcinoma were completely negative. HGF/SF mRNA extracted from a breast tumor demonstrated the expected 6-kb transcript on Northern blot analysis. These findings suggest the possibility of an autocrine loop for action of HGF/SF in proliferating mammary epithelium.
本研究通过原位杂交报告了生长调节因子肝细胞生长因子/分散因子（HGF/SF）的mRNA在人乳腺良恶性上皮中的表达情况。在良性乳腺组织中，腺病区域以及哺乳期乳腺的外周腺泡细胞中表达明显；在恶性上皮中，21例原位和浸润性乳腺癌中有15例可见表达。在一些浸润性导管癌病例中，与浸润性生长区域相比，较强的标记似乎与小管形成区域相关，尽管并非普遍现象。相比之下，原位粉刺癌和浸润性小叶癌各有两例完全阴性。从乳腺肿瘤中提取的HGF/SF mRNA在Northern印迹分析中显示出预期的6kb转录本。这些发现提示了HGF/SF在增殖性乳腺上皮中存在自分泌作用环路的可能性。
