Wang Y, Selden C, Farnaud S, Calnan D, Hodgson H J
Department of Medicine, Royal Postgraduate Medical School, London, UK.
J Anat. 1994 Dec;185 ( Pt 3)(Pt 3):543-51.
This study reports the tissue distribution of Hepatocyte Growth Factor-Scatter Factor (HGF/SF) in human fetal tissue using Northern analysis and in situ hybridisation techniques. In tissue from fetuses of 9-17 wk gestational age, the 6 kb mRNA transcript for HGF/SF was demonstrated in many tissues but prominently in liver, intestine, gall bladder and spleen. In situ hybridisation demonstrated that HGF/SF expression was not confined to mesenchymal tissues, as suggested by previous studies but was expressed in epithelial tissues, particularly in small intestine, keratinising epithelium of tongue, skin and oesophagus. In the small intestine epithelial expression was strikingly regional, being confined to the crypt region, the site of enterocyte proliferation. Northern analysis of tissues for c-met mRNA, representing expression of the HGF/SF receptor, demonstrated receptor expression in all tissues studied except the thyroid gland.
本研究运用Northern印迹分析和原位杂交技术，报告了肝细胞生长因子-分散因子（HGF/SF）在人胎儿组织中的组织分布情况。在妊娠9至17周胎儿的组织中，HGF/SF的6 kb mRNA转录本在许多组织中都有显示，但在肝脏、肠道、胆囊和脾脏中尤为显著。原位杂交表明，HGF/SF的表达并不局限于先前研究所提示的间充质组织，而是在上皮组织中表达，特别是在小肠、舌的角化上皮、皮肤和食管中。在小肠中，上皮表达具有显著的区域性，局限于隐窝区域，即肠上皮细胞增殖的部位。对代表HGF/SF受体表达的c-met mRNA进行组织的Northern印迹分析表明，除甲状腺外，在所研究的所有组织中均有受体表达。
