Takahashi M, Ota S, Terano A, Yoshiura K, Matsumura M, Niwa Y, Kawabe T, Nakamura T, Omata M
2nd Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Japan.
Biochem Biophys Res Commun. 1993 Mar 15;191(2):528-34. doi: 10.1006/bbrc.1993.1250.
Hepatocyte Growth Factor (HGF) was originally identified as hepatotrophic factor for liver regeneration. More recently, HGF was revealed to stimulate proliferation of various epithelial cells. At present study, we have investigated the mitogenic effect of HGF on gastric epithelial cells in the primary culture system. HGF remarkably stimulated proliferation of these cells dose dependently and synergistically with EGF and insulin. Mitogenic action of HGF might be mediated not by intracellular Ca2+ but by tyrosine kinase pathway. Conditioned medium of cultured fibroblast-like cells showed proliferative effects on cultured gastric epithelial cells in a similar way as exogenous HGF. These results suggest that HGF might be involved in the repair process of gastric mucosa.
肝细胞生长因子（HGF）最初被鉴定为肝脏再生的肝营养因子。最近，研究发现HGF能刺激各种上皮细胞的增殖。在本研究中，我们在原代培养系统中研究了HGF对胃上皮细胞的促有丝分裂作用。HGF能显著地剂量依赖性地刺激这些细胞的增殖，并与表皮生长因子（EGF）和胰岛素协同作用。HGF的促有丝分裂作用可能不是由细胞内钙离子介导的，而是由酪氨酸激酶途径介导的。培养的成纤维细胞样细胞的条件培养基对培养的胃上皮细胞具有与外源性HGF相似的增殖作用。这些结果表明，HGF可能参与胃黏膜的修复过程。
