Zhang Y, Hu Y, Wilson G S, Moatti-Sirat D, Poitout V, Reach G
Department of Chemistry, University of Kansas, Lawrence 66045.
Anal Chem. 1994 Apr 1;66(7):1183-8. doi: 10.1021/ac00079a038.
Acetaminophen has been one of the most serious electrochemical interferences to oxidase-based amperometric biosensors that measure H2O2. A study was carried out to investigate various polymer materials for their selectivity as the sensor inner membrane. A composite membrane of cellulose acetate and Nafion was found to eliminate acetaminophen and other electrochemical interferences effectively while at the same time maintaining reasonable diffusivity for hydrogen peroxide. The excellent in vivo performance of the sensor was attributed not only to significantly reduced steady-state sensitivity to acetaminophen but also to very slow acetaminophen response. These features, combined with rapid acetaminophen clearance pharmacokinetics, led to the decreased response as demonstrated in the rat.
对乙酰氨基酚一直是测量过氧化氢的基于氧化酶的安培型生物传感器最严重的电化学干扰之一。开展了一项研究，以研究各种聚合物材料作为传感器内膜的选择性。发现醋酸纤维素和Nafion的复合膜能有效消除对乙酰氨基酚和其他电化学干扰，同时保持过氧化氢合理的扩散性。该传感器出色的体内性能不仅归因于对乙酰氨基酚的稳态灵敏度显著降低，还归因于对乙酰氨基酚的响应非常缓慢。这些特性与对乙酰氨基酚快速清除的药代动力学相结合，导致了如在大鼠身上所显示的响应降低。
