体细胞杂交细胞系的进一步鉴定：牛基因组的十项新定位

Further characterization of a somatic cell hybrid panel: ten new assignments to the bovine genome.

作者信息

Guérin G, Eggen A, Vaiman D, Nocart M, Laurent P, Béchet D, Ferrara M

机构信息

Laboratoire de Génétique biochimique et de Cytogénétique, INRA-CRJ, Jouy-en-Josas, France.

出版信息

Anim Genet. 1994 Feb;25(1):31-5.

PMID:8161018

Abstract

Thirty-six partially characterized hamster-bovine hybrid cell lines were used for the determination of synteny groups. Sixteen additional reference loci, selected for their coverage of the bovine genome, were analysed on these hybrid cells. This increases to 25 the number of synteny groups detected. This panel was then used to make synteny assignments for 10 additional loci, eight by Southern blotting (COL1A1, COL1A2, FAS, CTSB, CTSL, CHRNG, HEXB and HTR1A) and two by polymerase chain reaction (PCR) amplification (HRH1 and ETH1112). These loci were assigned to international synteny groups U12 (HRH1), U13 (COL1A2), U17 (CHRNG), U21 (COL1A1, FAS), U29 (ETH1112), to chromosome 20 (U14 or U25) for HEXB and HTR1A, and to the same local synteny group (A), which is probably U18, for CTSB and CTSL. For three loci already mapped in humans (COL1A1, COL1A2 and CHRNG), the present results are in accordance with the predictions based on comparative mapping between the human and bovine species.

摘要

36个部分特征明确的仓鼠-牛杂交细胞系被用于确定同线群。在这些杂交细胞上分析了另外16个因其覆盖牛基因组而选择的参考位点。这使得检测到的同线群数量增加到25个。然后使用该细胞系对另外10个位点进行同线定位，其中8个通过Southern印迹法（COL1A1、COL1A2、FAS、CTSB、CTSL、CHRNG、HEXB和HTR1A），2个通过聚合酶链反应（PCR）扩增（HRH1和ETH1112）。这些位点被分配到国际同线群U12（HRH1）、U13（COL1A2）、U17（CHRNG）、U21（COL1A1、FAS）、U29（ETH1112），HEXB和HTR1A被分配到染色体20（U14或U25），CTSB和CTSL被分配到相同的局部同线群（A），可能是U18。对于已在人类中定位的3个位点（COL1A1、COL1A2和CHRNG），目前的结果与基于人类和牛物种之间比较图谱的预测一致。