Schwerin M, Solinas Toldo S, Eggen A, Brunner R, Seyfert H M, Fries R
Department of Molecular Biology, Research Institute for Biology of Farm Animals, Dummerstorf, Germany.
Mamm Genome. 1994 Aug;5(8):486-9. doi: 10.1007/BF00369317.
Five overlapping lambda EMBL-clones, containing the complete bovine lactoferrin gene (LTF), have been used to map this gene by fluorescence in situ hybridization to bovine Chromosome (Chr) band 22q24. Primers derived from promoter and exon I sequences were applied in polymerase chain reactions (PCRs) to DNA samples of a previously characterized panel of somatic cell hybrid lines, allowing the assignment of the bovine lactoferrin locus to syntenic group U12. These results permit the assignment of syntenic group U12 to bovine Chr 22.
五个重叠的λEMBL克隆包含完整的牛乳铁蛋白基因(LTF),已通过荧光原位杂交将该基因定位到牛染色体(Chr)22q24带。从启动子和外显子I序列衍生的引物应用于一组先前已鉴定的体细胞杂交系的DNA样本的聚合酶链反应(PCR)中,从而将牛乳铁蛋白基因座定位到同线群U12。这些结果允许将同线群U12定位到牛Chr 22。