Schild D, Lischka F W
Physiologisches Institut, Universität Göttingen, Germany.
Biophys J. 1994 Feb;66(2 Pt 1):299-304. doi: 10.1016/s0006-3495(94)80804-5.
We used digital calcium imaging with Fura-2 in conjunction with the tight-seal whole-cell patch clamp technique to describe a novel cation conductance in olfactory neurons of the clawed toad Xenopus laevis. Substitution of extracellular Ca2+ and Na+ was used as a tool to change [Ca2+]i. When [Ca2+]i was increased to about 450 nM, a conductance gcat activated that was permeable for cations. Upon gcat activation, an increase in [Ca2+]i occurred in the dendritic knob. Once activated, gcat showed no further dependence upon [Ca2+]i. Icat is shown to be different from the current activated by a mixture of the odorants citralva and amyl acetate. We conclude that there are two different cation conductances in the peripheral compartments of olfactory neurons in X. laevis.
我们运用结合了紧密密封全细胞膜片钳技术的Fura-2数字钙成像，来描述非洲爪蟾（Xenopus laevis）嗅觉神经元中的一种新型阳离子电导。用细胞外Ca2+和Na+的替代物作为改变[Ca2+]i的工具。当[Ca2+]i增加到约450 nM时，一种对阳离子通透的电导gcat被激活。gcat激活后，树突小体中的[Ca2+]i增加。一旦被激活，gcat不再进一步依赖[Ca2+]i。Icat被证明不同于由柠檬醛和乙酸戊酯混合物激活的电流。我们得出结论，非洲爪蟾嗅觉神经元外周区存在两种不同的阳离子电导。
