Stofer E, Lavery R
Laboratoire de Biochimie Théorique (CNRS URA 77), Institut de Biologie Physico-Chimique, Paris, France.
Biopolymers. 1994 Mar;34(3):337-46. doi: 10.1002/bip.360340305.
We present a new method for measuring the widths and depths of the grooves formed within DNA helices. This method overcomes the limitations of simply measuring interstrand phosphate-phosphate distances and has the advantage of yielding continuous values for groove geometry along a DNA fragment. In the case of oligonucleotides, it also clearly indicates the zones in which grooves exist, bounded by two phosphodiester backbones. The methodology has been developed within the Curves algorithm for studying irregular DNA geometries and is based on the optimal, and generally curved, helical axis obtained by this analysis.
我们提出了一种测量DNA螺旋内部形成的凹槽宽度和深度的新方法。该方法克服了简单测量链间磷酸-磷酸距离的局限性，具有沿DNA片段产生凹槽几何形状连续值的优点。对于寡核苷酸，它还清楚地表明了由两个磷酸二酯主链界定的凹槽存在区域。该方法是在用于研究不规则DNA几何形状的Curves算法中开发的，并且基于通过该分析获得的最佳且通常为弯曲的螺旋轴。
