刘易斯大鼠的眼压

Intraocular pressure in Lewis rats.

作者信息

Mermoud A, Baerveldt G, Minckler D S, Lee M B, Rao N A

机构信息

Doheny Eye Institute, Los Angeles, CA 90033.

出版信息

Invest Ophthalmol Vis Sci. 1994 Apr;35(5):2455-60.

PMID:8163335

Abstract

PURPOSE

To perform noninvasive measurements of intraocular pressure (IOP) in rats, the Tono-Pen-1 and Tono-Pen-2 were calibrated against direct manometry. Normal values and the long-term fluctuations of IOP in Lewis rats were established.

METHODS

For calibration, 24 eyes were cannulated and connected to a pressure transducer with a chart recorder. IOP was increased from 5 to 40 mm Hg in 5 mm Hg increments, and from 40 to 60 mm Hg in 10 mm Hg increments. After each incremental increase, IOP was measured with a Tono-Pen-1 and a Tono-Pen-2 tonometer. To determine normal IOP in Lewis rats, IOP was measured with a Tono-Pen-1 in 229 eyes of 115 rats, and a histogram of normal IOP was established. To ascertain long-term IOP fluctuations, the pressure in 52 eyes of 26 rats was measured every day between 8:30 and 9:30 AM for 7 consecutive days.

RESULTS

Plotting the mean Tono-Pen readings for each eye against the transducer IOP produced two regression formulas: y = 1.819 + 0.711 x (r2 = 0.92) for Tono-Pen-1, and y = -1.291 + 0.784 x (r2 = 0.97) for Tono-Pen-2. The normal IOP in rats was 17.30 +/- 5.25 mm Hg (90% confidence interval: 7.28 and 26.98 mm Hg for the lower and upper limits of normal IOP). There was no long-term fluctuation in IOP (P = 0.55).

CONCLUSIONS

IOP can be measured accurately in living rats with the Tono-Pen-1 or the Tono-Pen-2.

摘要

目的

为了对大鼠眼压（IOP）进行无创测量，将Tono - Pen - 1眼压计和Tono - Pen - 2眼压计与直接测压法进行校准。确定了Lewis大鼠眼压的正常值及其长期波动情况。

方法

为进行校准，对24只眼睛进行插管，并连接到带有图表记录器的压力传感器上。眼压以5 mmHg的增量从5 mmHg升高到40 mmHg，然后以10 mmHg的增量从40 mmHg升高到60 mmHg。每次增量升高后，使用Tono - Pen - 1眼压计和Tono - Pen - 2眼压计测量眼压。为确定Lewis大鼠的正常眼压，使用Tono - Pen - 1对115只大鼠的229只眼睛进行眼压测量，并建立正常眼压的直方图。为确定眼压的长期波动情况，在连续7天的每天上午8:30至9:30之间，对26只大鼠的52只眼睛的眼压进行测量。

结果

将每只眼睛的Tono - Pen平均读数与传感器测量的眼压进行绘图，得出两个回归公式：Tono - Pen - 1的公式为y = 1.819 + 0.711x（r2 = 0.92），Tono - Pen - 2的公式为y = -1.291 + 0.784x（r2 = 0.97）。大鼠的正常眼压为17.30±5.25 mmHg（90%置信区间：正常眼压下限为7.28 mmHg，上限为26.98 mmHg）。眼压没有长期波动（P = 0.55）。