TGN38糖蛋白包含两个不重叠的信号，这些信号介导其定位于反式高尔基体网络。
The TGN38 glycoprotein contains two non-overlapping signals that mediate localization to the trans-Golgi network.
作者信息
Ponnambalam S, Rabouille C, Luzio J P, Nilsson T, Warren G
机构信息
Cell Biology Laboratory, Imperial Cancer Research Fund, Lincoln's Inn Fields, London, United Kingdom.
出版信息
J Cell Biol. 1994 Apr;125(2):253-68. doi: 10.1083/jcb.125.2.253.
Abstract
The membrane-spanning and cytoplasmic domains of CD4 and CD8 were replaced by those of TGN38. After transient expression in HeLa cells, the location of the hybrid proteins was determined using immunofluorescence and quantitative immuno-electron microscopy, FACS analysis and metabolic labeling. The membrane-spanning domain was found to contain a signal that localized hybrid proteins to the TGN. This was in addition to the signal previously identified in the cytoplasmic domain (Bos, K., C. Wraight, and K. Stanley. 1993. EMBO (Eur. Mol. Biol. Organ) J. 12:2219-2228. Humphrey, J. S., P. J. Peters, L. C. Yuan, and J. S. Bonifacino. 1993. J. Cell Biol. 120:1123-1135. Wong, S. H., and W. Hong. 1993. J. Biol. Chem. 268:22853-22862). The different properties of these two signals suggest that each operates by a different mechanism.
摘要
CD4和CD8的跨膜结构域及胞质结构域被TGN38的相应结构域所取代。在HeLa细胞中瞬时表达后，利用免疫荧光、定量免疫电子显微镜、流式细胞术分析及代谢标记确定了杂交蛋白的定位。发现跨膜结构域含有一个将杂交蛋白定位到反式高尔基体网络（TGN）的信号。这是除了先前在胞质结构域中鉴定出的信号之外的信号（博斯，K.，C. 赖特，和K. 斯坦利。1993年。《欧洲分子生物学组织杂志》12:2219 - 2228。汉弗莱，J. S.，P. J. 彼得斯，L. C. 袁，和J. S. 博尼法西诺。1993年。《细胞生物学杂志》120:1123 - 1135。黄，S. H.，和W. 洪。1993年。《生物化学杂志》268:22853 - 228,62）。这两种信号的不同特性表明它们各自通过不同的机制发挥作用。
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本文引用的文献
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Golgi retention signals: do membranes hold the key?高尔基体保留信号：膜是关键所在吗？
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TGN38 is maintained in the trans-Golgi network by a tyrosine-containing motif in the cytoplasmic domain.TGN38通过其胞质结构域中含酪氨酸的基序维持在内质网反式高尔基体网络中。
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TGN38/41 recycles between the cell surface and the TGN: brefeldin A affects its rate of return to the TGN.TGN38/41在细胞表面和反式高尔基体网络（TGN）之间循环：布雷菲德菌素A会影响其返回TGN的速率。
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