Nilsson T, Pypaert M, Hoe M H, Slusarewicz P, Berger E G, Warren G
Cell Biology Laboratory, Imperial Cancer Research Fund, London, United Kingdom.
J Cell Biol. 1993 Jan;120(1):5-13. doi: 10.1083/jcb.120.1.5.
Thin, frozen sections of a HeLa cell line were double labeled with specific antibodies to localize the trans-Golgi enzyme, beta 1,4 galactosyltransferase (GalT) and the medial enzyme, N-acetylglucosaminyltransferase I (NAGT I). The latter was detected by generating a HeLa cell line stably expressing a myc-tagged version of the endogenous protein. GalT was found in the trans-cisterna and trans-Golgi network but, contrary to expectation, NAGT I was found both in the medial- and trans-cisternae, overlapping the distribution of GalT. About one third of the NAGT I and half of the GalT were found in the shared, trans-cisterna. These data show that the differences between cisternae are determined not by different sets of enzymes but by different mixtures.
将HeLa细胞系的薄冰冻切片用特异性抗体进行双重标记，以定位反式高尔基体酶β1,4半乳糖基转移酶（GalT）和中间酶N-乙酰葡糖胺基转移酶I（NAGT I）。通过构建稳定表达内源性蛋白的myc标签版本的HeLa细胞系来检测后者。GalT存在于反式扁平囊和反式高尔基体网络中，但与预期相反，NAGT I在内侧扁平囊和反式扁平囊中均有发现，其分布与GalT重叠。约三分之一的NAGT I和一半的GalT存在于共同的反式扁平囊中。这些数据表明，扁平囊之间的差异不是由不同的酶组决定的，而是由不同的混合物决定的。
