两种糖基转移酶在HeLa细胞高尔基体中的重叠分布。
Overlapping distribution of two glycosyltransferases in the Golgi apparatus of HeLa cells.
作者信息
Nilsson T, Pypaert M, Hoe M H, Slusarewicz P, Berger E G, Warren G
机构信息
Cell Biology Laboratory, Imperial Cancer Research Fund, London, United Kingdom.
出版信息
J Cell Biol. 1993 Jan;120(1):5-13. doi: 10.1083/jcb.120.1.5.
Abstract
Thin, frozen sections of a HeLa cell line were double labeled with specific antibodies to localize the trans-Golgi enzyme, beta 1,4 galactosyltransferase (GalT) and the medial enzyme, N-acetylglucosaminyltransferase I (NAGT I). The latter was detected by generating a HeLa cell line stably expressing a myc-tagged version of the endogenous protein. GalT was found in the trans-cisterna and trans-Golgi network but, contrary to expectation, NAGT I was found both in the medial- and trans-cisternae, overlapping the distribution of GalT. About one third of the NAGT I and half of the GalT were found in the shared, trans-cisterna. These data show that the differences between cisternae are determined not by different sets of enzymes but by different mixtures.
摘要
将HeLa细胞系的薄冰冻切片用特异性抗体进行双重标记，以定位反式高尔基体酶β1,4半乳糖基转移酶（GalT）和中间酶N-乙酰葡糖胺基转移酶I（NAGT I）。通过构建稳定表达内源性蛋白的myc标签版本的HeLa细胞系来检测后者。GalT存在于反式扁平囊和反式高尔基体网络中，但与预期相反，NAGT I在内侧扁平囊和反式扁平囊中均有发现，其分布与GalT重叠。约三分之一的NAGT I和一半的GalT存在于共同的反式扁平囊中。这些数据表明，扁平囊之间的差异不是由不同的酶组决定的，而是由不同的混合物决定的。
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本文引用的文献
1
The use of lead citrate at high pH as an electron-opaque stain in electron microscopy.在电子显微镜检查中，将高pH值的柠檬酸铅用作电子不透明染色剂。
J Cell Biol. 1963 Apr;17(1):208-12. doi: 10.1083/jcb.17.1.208.
2
Antibodies to the Golgi complex and the rough endoplasmic reticulum.针对高尔基体和粗面内质网的抗体。
J Cell Biol. 1982 Jan;92(1):92-107. doi: 10.1083/jcb.92.1.92.
3
Immunoelectron microscopic exploration of the Golgi complex.高尔基体的免疫电子显微镜研究。
J Histochem Cytochem. 1983 Aug;31(8):1049-56. doi: 10.1177/31.8.6863900.
4
Early and late functions associated with the Golgi apparatus reside in distinct compartments.与高尔基体相关的早期和晚期功能存在于不同的区室中。
Proc Natl Acad Sci U S A. 1981 Dec;78(12):7453-7. doi: 10.1073/pnas.78.12.7453.
5
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Proc Natl Acad Sci U S A. 1983 Jul;80(14):4364-8. doi: 10.1073/pnas.80.14.4364.
6
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Cell. 1984 Dec;39(2 Pt 1):405-16. doi: 10.1016/0092-8674(84)90019-9.
7
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8
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9
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J Cell Biol. 1983 Sep;97(3):723-7. doi: 10.1083/jcb.97.3.723.
10
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