光刺激下叶绿体DNA的合成。

Light-stimulated synthesis of chloroplast DNA.

作者信息

Stolarsky L, Walfield A M, Birch R A, Hershberger C L

出版信息

Biochim Biophys Acta. 1976 Apr 2;425(4):438-50. doi: 10.1016/0005-2787(76)90008-3.

Abstract

Light-stimulated chloroplast DNA synthesis was studied during chloroplast development in the absence of cell division and nuclear DNA synthesis. Incorporation of 32Pi was stimulated 10-15 fold, however, the ratio of chloroplast DNA to nuclear DNA remained constant. Isotope dilution experiments suggested that stimulated labeling of chloroplast DNA represented more efficient utilization of exogenously supplied Pi rather than stimulated turnover of chloroplast DNA. The low level of DNA synthesis and chloroplast development were resistant to nalidixic acid which inhibits semiconservative replication of chloroplast DNA.

摘要

在没有细胞分裂和核DNA合成的情况下，研究了叶绿体发育过程中光刺激的叶绿体DNA合成。32Pi的掺入增加了10 - 15倍，然而，叶绿体DNA与核DNA的比例保持恒定。同位素稀释实验表明，叶绿体DNA的刺激标记代表了对外源供应Pi的更有效利用，而不是叶绿体DNA的刺激周转。低水平的DNA合成和叶绿体发育对抑制叶绿体DNA半保留复制的萘啶酸具有抗性。