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叶绿体DNA编码转运RNA。

Chloroplast DNA codes for transfer RNA.

作者信息

McCrea J M, Hershberger C L

出版信息

Nucleic Acids Res. 1976 Aug;3(8):2005-18. doi: 10.1093/nar/3.8.2005.

DOI:10.1093/nar/3.8.2005
PMID:823529
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC343056/
Abstract

Transfer RNA's were isolated from Euglena gracilis. Chloroplast cistrons for tRNA were quantitated by hybridizing tRNA to ct DNA. Species of tRNA hybridizing to ct DNA were partially purified by hybridization-chromatography. The tRNA's hybridizing to ct DNA and nuclear DNA appear to be different. Total cellular tRNA was hybridized to ct DNA to an equivalent of approximately 25 cistrons. The total cellular tRNA was also separated into 2 fractions by chromatography on dihydroxyboryl substituted amino ethyl cellulose. Fraction I hybridized to both nuclear and ct DNA. Hybridizations to ct DNA indicated approximately 18 cistrons. Fraction II-tRNA hybridized only to ct DNA, saturating at a level of approximately 7 cistrons. The tRNA from isolated chloroplasts hybridized to both chloroplast and nuclear DNA. The level of hybridization to ct DNA indicated approximately 18 cistrons. Fraction II-type tRNA could not be detected in the isolated chloroplasts.

摘要

从纤细裸藻中分离出了转运核糖核酸（tRNA）。通过使tRNA与叶绿体DNA（ct DNA）杂交来定量叶绿体中tRNA的顺反子。通过杂交色谱法对与ct DNA杂交的tRNA种类进行了部分纯化。与ct DNA和核DNA杂交的tRNA似乎有所不同。总的细胞tRNA与ct DNA杂交，相当于大约25个顺反子。总的细胞tRNA也通过在二羟基硼基取代的氨乙基纤维素上进行色谱分离成两个部分。部分I与核DNA和ct DNA都杂交。与ct DNA的杂交表明大约有18个顺反子。部分II - tRNA仅与ct DNA杂交，在大约7个顺反子的水平达到饱和。从分离出的叶绿体中提取的tRNA与叶绿体DNA和核DNA都杂交。与ct DNA的杂交水平表明大约有18个顺反子。在分离出的叶绿体中未检测到部分II型tRNA。

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本文引用的文献

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