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对眼虫细胞核和叶绿体中稳定RNA的基因进行计数。

Counting the genes for stabel RNA in the nucleus and chloroplasts of Euglena.

作者信息

Gruol D J, Haselkorn R

出版信息

Biochim Biophys Acta. 1976 Sep 20;447(1):82-95. doi: 10.1016/0005-2787(76)90098-8.

DOI:10.1016/0005-2787(76)90098-8
PMID:822884
Abstract

Improved procedures for counting the genes for ribosomal and 4 S RNA in the nucleus and chloroplasts of Euglena are described. These procedures exploit (a) the chloroplast-free strain ZHB, (b) the flotation method for purifying chloroplasts, (c) Hg2+/Cs2SO4 density gradients to fractionate large quantities of DNA and (d) more efficient conditions of DNA - RNA hybridization particularly for 4 S RNA. We find that the average Euglena cell in an exponentially growing culture contains 800 nuclear genes for each cytoplasmic rRNA and 800-880 chloroplast genes for chloroplast rRNA. Each chloroplast chromosome of molecular weight 92 - 10(6) contains two genes for each rRNA. The number of nuclear genes for 4 S RNA is approx. 760. The total number of chloroplast genes for 4S RNA is approx. 10 000, or 22-25 on each chloroplast chromosome.

摘要

本文描述了用于计算眼虫细胞核和叶绿体中核糖体RNA和4S RNA基因数量的改进方法。这些方法利用了：（a）无叶绿体菌株ZHB；（b）纯化叶绿体的浮选法；（c）Hg2+/Cs2SO4密度梯度法来分离大量DNA；（d）更有效的DNA-RNA杂交条件，特别是针对4S RNA的条件。我们发现，在指数生长培养中的平均眼虫细胞，每个细胞质rRNA含有800个核基因，叶绿体rRNA含有800 - 880个叶绿体基因。每个分子量为92 - 10(6)的叶绿体染色体，每个rRNA含有两个基因。4S RNA的核基因数量约为760个。4S RNA的叶绿体基因总数约为10000个，即每个叶绿体染色体上有22 - 25个。

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引用本文的文献

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Transfer RNA genes ofZea mays chloroplast DNA.玉米叶绿体 DNA 的转移 RNA 基因。
Plant Mol Biol. 1983 May;2(3):141-53. doi: 10.1007/BF01578514.
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Fractionation and identification of Euglena gracilis cytoplasmic and chloroplastic tRNAs and mapping of tRNA genes on chloroplast DNA.小球藻细胞质和叶绿体 tRNA 的分离与鉴定及叶绿体 DNA 上 tRNA 基因的定位。
Curr Genet. 1982 Oct;6(1):63-9. doi: 10.1007/BF00397643.
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Analysis of intergenic spacer transcripts suggests 'read-around' transcription of the extrachromosomal circular rDNA in Euglena gracilis.基因间间隔转录本分析表明纤细裸藻中染色体外环状rDNA存在“通读”转录。
Nucleic Acids Res. 2001 May 15;29(10):2191-8. doi: 10.1093/nar/29.10.2191.
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Structure and evolution of organelle genomes.细胞器基因组的结构与进化
Microbiol Rev. 1982 Jun;46(2):208-40. doi: 10.1128/mr.46.2.208-240.1982.
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The nucleotide sequences of the genes coding for tRNAArgUCU, tRNAArgACG and tRNAAsnGUU on Spirodela oligorhiza chloroplast DNA.紫萍叶绿体DNA上编码tRNAArgUCU、tRNAArgACG和tRNAAsnGUU的基因的核苷酸序列。
Nucleic Acids Res. 1984 Jul 25;12(14):5639-46. doi: 10.1093/nar/12.14.5639.
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Extrachromosomal circular nuclear rDNA in Euglena gracilis.纤细裸藻中的染色体外环状核rDNA。
Nucleic Acids Res. 1985 Oct 25;13(20):7529-37. doi: 10.1093/nar/13.20.7529.
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Nuclear rDNA in Euglena gracilis: paucity of chromosomal units and replication of extrachromosomal units.纤细裸藻中的核核糖体DNA：染色体单元数量稀少及染色体外单元的复制
Nucleic Acids Res. 1988 Jun 10;16(11):4801-10. doi: 10.1093/nar/16.11.4801.

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