Carballido J M, Carballido-Perrig N, Oberli-Schrämmli A, Heusser C H, Blaser K
Swiss Institute of Allergy and Asthma Research, Davos.
J Allergy Clin Immunol. 1994 Apr;93(4):758-67. doi: 10.1016/0091-6749(94)90256-9.
An in vitro antibody response to bee venom phospholipase A2 (PLA) from peripheral blood mononuclear cells of bee sting-sensitized individuals was achieved after stimulation with PLA and pokeweed mitogen. This stimulation resulted in a secretion of TH1-associated cytokines and induced PLA-specific and nonspecific IgG4 antibody production but not IgE production. The addition of interleukin-4 (IL-4) to this system decreased the secretion of IgG antibodies, whereas secretion of polyspecific IgE was induced. The mitogen was not required if peripheral blood mononuclear cells were enriched with autologous, PLA-specific, resting T-cell clones in the presence of the antigen. In these experiments the cytokine profile of the particular clone determined the antibody class generated. Low ratios of IL-4 to interferon-gamma, induced by the antigen alone or obtained by neutralizing anti-IL-4 antibodies, enhanced IgG4 antibody formation, whereas IgE levels increased at high ratios of IL-4 to interferon-gamma. These results suggest a complementary regulation of the main isotypes, IgE and IgG4, implicated in allergic and protective hyperimmune responses.
在用蜂毒磷脂酶A2（PLA）和商陆有丝分裂原刺激后，实现了对蜂蛰致敏个体外周血单个核细胞的体外抗体反应。这种刺激导致TH1相关细胞因子的分泌，并诱导产生PLA特异性和非特异性IgG4抗体，但不产生IgE。向该系统中添加白细胞介素-4（IL-4）会减少IgG抗体的分泌，而诱导产生多特异性IgE的分泌。如果在存在抗原的情况下，用自体的、PLA特异性的静息T细胞克隆富集外周血单个核细胞，则不需要有丝分裂原。在这些实验中，特定克隆的细胞因子谱决定了产生的抗体类别。单独由抗原诱导或通过中和抗IL-4抗体获得的低IL-4与干扰素-γ比值，增强了IgG4抗体的形成，而在高IL-4与干扰素-γ比值时IgE水平升高。这些结果表明，在过敏和保护性超免疫反应中，主要同种型IgE和IgG4存在互补调节。
