Harshey R M, Ramakrishnan T
Biochim Biophys Acta. 1976 Apr 15;432(1):49-59. doi: 10.1016/0005-2787(76)90040-x.
RNA polymerase (nucleosidetriphosphate: RNA nucleotidyltransferase DNA-dependent), EC 2.7.7.6) was purified approximately 200 fold from Mycobacterium tuberculosis H37RV cells. The purified enzyme has a molecular weight of about 330 000-350 000 and is composed of four subunits. The subunits beta', beta and sigma have molecular weights different from those of Escherichia coli polymerase; the fourth, alpha subunit has a similar weight. The purified enzyme is a thousand-fold more sensitive to rifampicin, a potent antitubercular drug than the E. coli RNA polymerase, probably because of the difference in the beta subunits. This, with other data presented in this paper, indicate that the RNA polymerase of M. tuberculosis differs in its properties from that of E. coli.
RNA聚合酶（核苷三磷酸：RNA核苷酸基转移酶，依赖DNA），EC 2.7.7.6）从结核分枝杆菌H37RV细胞中纯化出来，纯化倍数约为200倍。纯化后的酶分子量约为330000 - 350000，由四个亚基组成。β'、β和σ亚基的分子量与大肠杆菌聚合酶的不同；第四个α亚基的分子量相似。纯化后的酶对强力抗结核药物利福平的敏感性比大肠杆菌RNA聚合酶高一千倍，这可能是由于β亚基的差异。本文给出的其他数据表明，结核分枝杆菌的RNA聚合酶在性质上与大肠杆菌的不同。
