Takihara H, Yoshida M, Aritomi T, Toyoshima H, Nakanishi M, Ishibashi M, Watanabe K
School of Medicine, Fukuoka University, Japan.
Nihon Kyobu Shikkan Gakkai Zasshi. 1994 Feb;32(2):130-7.
The effects of repeated intravenous injections of E. coli endotoxin (ETX)-containing liposomes (LP) on the development of emphysematous change in rats were examined. Male Wistar rats, weighting 150-200 g, were divided into 3 groups. Group 1; rats treated with phosphate buffered saline (PBS), Group 2; rats treated with liposome-entrapped PBS, Group 3; rats treated with liposome-entrapped ETX (10 mg/ml). In each group, 0.5 ml was injected intravenously once a week for 8 consecutive weeks. After 8 weeks, functional and morphometrical analyses of lungs were performed. Functional residual capacity (FRC), total lung capacity (TLC), and static compliance (Cst.) were measured using a pressure plethysmograph for small animals. Mean linear intercept (MLI) and internal surface area (ISA) were also determined. TLC did not differ among the 3 groups. In rats treated with liposome-entrapped ETX, Cst. corrected by body weight (Cst./body weight) was significantly increased compared with that in liposome-entrapped PBS-treated rats. Further more, MLI was larger than that of the other 2 groups and ISA was less than that of the liposome-entrapped PBS-treated rats. These results suggest that liposome-entrapped ETX induces emphysematous changes in rats.
研究了反复静脉注射含大肠杆菌内毒素（ETX）的脂质体（LP）对大鼠肺气肿样改变发展的影响。将体重150 - 200克的雄性Wistar大鼠分为3组。第1组：用磷酸盐缓冲盐水（PBS）处理的大鼠；第2组：用包裹PBS的脂质体处理的大鼠；第3组：用包裹ETX（10毫克/毫升）的脂质体处理的大鼠。每组每周静脉注射0.5毫升，连续注射8周。8周后，对肺进行功能和形态学分析。使用小动物压力体积描记法测量功能残气量（FRC）、肺总量（TLC）和静态顺应性（Cst.）。还测定了平均线性截距（MLI）和内表面积（ISA）。3组之间TLC无差异。在用包裹ETX的脂质体处理的大鼠中，经体重校正的Cst.（Cst./体重）与用包裹PBS的脂质体处理的大鼠相比显著增加。此外，MLI大于其他两组，ISA小于用包裹PBS的脂质体处理的大鼠。这些结果表明，包裹ETX的脂质体可诱导大鼠发生肺气肿样改变。
