Simonsen L, Bülow J, Madsen J
Department of Clinical Physiology, Hvidovre Hospital, Copenhagen, Denmark.
Am J Physiol. 1994 Mar;266(3 Pt 1):E357-65. doi: 10.1152/ajpendo.1994.266.3.E357.
A technique for catheterization of a vein draining abdominal subcutaneous tissue and a microdialysis technique that allows measurements of intercellular water concentrations in adipose tissue in humans have recently been described. In the present study, we compare the two techniques during an oral glucose load. In addition a technique using microdialysis for measurement of tissue oxygen and carbon dioxide tensions is described. Microdialysis and vein catheterization were performed in the same region on the abdomen, and the subcutaneous adipose tissue blood flow was measured by the local 133Xe washout method. The results show that subcutaneous adipose tissue gas tensions are on level with gas tensions measured in abdominal venous blood. Comparison of metabolite concentrations measured in the venous blood and venous blood concentrations calculated from microdialysis data shows that there is good agreement between the concentrations obtained by the two techniques with respect to glucose and glycerol, whereas lactate concentrations are very different. With regard to substrate fluxes calculated by Fick's principle, the catheterization technique is probably the most reliable, considering the numerous assumptions on which calculations of venous concentrations from microdialysis data are based. Advantages and disadvantages of the two techniques are discussed.
最近描述了一种用于引流腹部皮下组织静脉的插管技术以及一种能够测量人体脂肪组织细胞间水浓度的微透析技术。在本研究中，我们比较了口服葡萄糖负荷期间的这两种技术。此外，还描述了一种使用微透析测量组织氧分压和二氧化碳分压的技术。在腹部同一区域进行微透析和静脉插管，并通过局部¹³³Xe洗脱法测量皮下脂肪组织血流量。结果表明，皮下脂肪组织的气体分压与腹部静脉血中测得的气体分压处于同一水平。对静脉血中测得的代谢物浓度与根据微透析数据计算出的静脉血浓度进行比较，结果显示，两种技术在葡萄糖和甘油浓度方面获得的结果具有良好的一致性，而乳酸浓度则差异很大。关于通过菲克原理计算的底物通量，考虑到根据微透析数据计算静脉浓度所基于的众多假设，插管技术可能是最可靠的。讨论了这两种技术的优缺点。
