Guo D F, Furuta H, Mizukoshi M, Inagami T
Department of Biochemistry, Vanderbilt University, School of Medicine, Nashville, TN 37232.
Biochem Biophys Res Commun. 1994 Apr 15;200(1):313-9. doi: 10.1006/bbrc.1994.1450.
As a step toward the elucidation of human Ang II type 1 receptor gene expression, the genomic organization of the human AT1 receptor was investigated. Comparison of the genomic DNA and cDNA sequences revealed that it consists of at least five exons. The length of the AT1 receptor gene is greater than 55 kb, and the size of the exons ranges from 59 to 2,014 base pairs. Four of the exons encoded 5'-untranslated sequences. Multiple transcription initiation sites were observed by primer extension experiment. The promoter function was examined by using luciferase as a report gene in transfected human vascular smooth muscle cells.
作为阐明人类血管紧张素II 1型受体基因表达的第一步，对人类AT1受体的基因组结构进行了研究。基因组DNA和cDNA序列的比较显示，它至少由五个外显子组成。AT1受体基因的长度大于55 kb，外显子大小从59到2014个碱基对不等。其中四个外显子编码5'-非翻译序列。通过引物延伸实验观察到多个转录起始位点。在转染的人血管平滑肌细胞中，以荧光素酶作为报告基因检测启动子功能。
