Martin M M, Elton T S
Vascular Biology and Hypertension Program, University of Alabama at Birmingham 35294, USA.
Biochem Biophys Res Commun. 1995 Apr 17;209(2):554-62. doi: 10.1006/bbrc.1995.1537.
A human genomic DNA library was screened utilizing a human angiotensin II type 2 receptor (hAT2R) cDNA as a probe. Several positive clones were isolated and characterized. A comparison of the hAT2R cDNA sequence with the hAT2R genomic clone sequence suggests that the hAT2R gene is composed of three exons and spans at least 5 kb. Exons 1 and 2 encode for 5' untranslated mRNA sequence and exon 3 harbors the entire uninterrupted open reading frame of the hAT2R. Sequence analysis of the 5'-flanking region of the hAT2R gene demonstrates that it contains the typical sequence motifs found in many eukaryotic promoters. Interestingly, however, this promoter region also includes an interferon consensus sequence binding protein site (ICSBP) and a putative embryonal, long terminal repeat binding protein (ELP) site. The presence of these novel putative transcription factor binding sites suggests that this gene may be regulated in a unique manner.
利用人血管紧张素II 2型受体（hAT2R）cDNA作为探针筛选人基因组DNA文库。分离并鉴定了几个阳性克隆。hAT2R cDNA序列与hAT2R基因组克隆序列的比较表明，hAT2R基因由三个外显子组成，跨度至少5 kb。外显子1和2编码5'非翻译mRNA序列，外显子3包含hAT2R的整个不间断开放阅读框。hAT2R基因5'侧翼区域的序列分析表明，它包含许多真核启动子中发现的典型序列基序。然而，有趣的是，该启动子区域还包括一个干扰素共有序列结合蛋白位点（ICSBP）和一个假定的胚胎长末端重复结合蛋白（ELP）位点。这些新的假定转录因子结合位点的存在表明该基因可能以独特的方式受到调控。
