Tashiro S, Michiue T, Higashijima S, Zenno S, Ishimaru S, Takahashi F, Orihara M, Kojima T, Saigo K
Department of Biophysics and Biochemistry, Faculty of Science, University of Tokyo, Japan.
Gene. 1993 Feb 28;124(2):183-9. doi: 10.1016/0378-1119(93)90392-g.
The complete nucleotide sequence of the coding region of hedgehog (hh), a segment-polarity gene in Drosophila melanogaster, was determined. The gene was found to include three exons which would encode a 421- (or 471-) amino acid (aa) polypeptide with a long hydrophobic stretch. The hh mRNA was about 2.3 kb long and expressed throughout development. The hh expression in an embryo occurred in stripes, while that in imaginal discs occurred in the posterior compartment. As a whole, the spatial expression pattern of hh mRNA was very similar to that of engrailed (en), a homeobox gene required for the formation of the anterior-posterior compartment boundary. Unlike en, no hh expression was observed in the central nervous system.
测定了果蝇中一个体节极性基因刺猬（hh）编码区的完整核苷酸序列。发现该基因包含三个外显子，它们将编码一个含有长疏水区域的421（或471）个氨基酸的多肽。hh mRNA约2.3 kb长，在整个发育过程中均有表达。hh在胚胎中的表达呈条纹状，而在成虫盘的表达则出现在后区室。总体而言，hh mRNA的空间表达模式与engrailed（en）非常相似，engrailed是形成前后区室边界所需的一个同源异型框基因。与en不同，在中枢神经系统中未观察到hh的表达。
