Heins S, Aebi U
Biocenter, University of Basel, Switzerland.
Curr Opin Cell Biol. 1994 Feb;6(1):25-33. doi: 10.1016/0955-0674(94)90112-0.
Thus far, intermediate filaments (IFs) have been the least understood of the three cytoskeletal filament systems with regard to their structure, assembly, network formation, and dynamics. This picture is now slowly but definitely changing, as recent in vivo and in vitro experiments, including generation of transgenic animals, have yielded important new data shedding light on the following areas: the molecular architecture of IFs; the role of the highly variable end domains during IF assembly and network formation; the factors that govern whether IF proteins are involved in de novo filament formation or are incorporated into a pre-existing IF network; and the effects of post-translational modifications, such as phosphorylation and glycosylation of IF polypeptides, on filament assembly, dynamics and turnover.
迄今为止，就中间丝（IFs）的结构、组装、网络形成和动力学而言，它们是三种细胞骨架丝系统中了解最少的。随着包括转基因动物的产生在内的近期体内和体外实验在以下领域产生了重要的新数据，这种情况现在正在缓慢但肯定地发生变化：中间丝的分子结构；高度可变的末端结构域在中间丝组装和网络形成过程中的作用；决定中间丝蛋白是参与从头形成丝还是并入预先存在的中间丝网络的因素；以及翻译后修饰，如中间丝多肽的磷酸化和糖基化，对丝组装、动力学和周转的影响。
