Polynuclear fertilization in various stimulation protocols in in vitro fertilization.

OBJECTIVE

To investigate the effect of some clinical characteristics, especially various ovarian stimulation protocols, on the occurrence of polypronuclear fertilization.

METHODS

One hundred forty-eight consecutive cycles in 121 patients were treated in our IVF program and 735 oocytes were analyzed retrospectively.

RESULTS

Multipronuclear fertilization occurred in 33 of 530 fertilized oocytes, an incidence of 6.2%. No significant difference in the incidence of polyploidy was observed between the three stimulation protocols (CC/hMG, hMG, GnRHa/hMG). Number of polypronuclear embryos was found to be correlated with the fertilization rate, concentration of sperm inseminated, and maturity of the oocytes.

CONCLUSIONS

The maturity of the oocytes and the sperm fertilizing capacity should be examined to reduce the incidence of polypronuclear fertilization.