Uhler M L, Buyalos R P
Department of Obstetrics and Gynecology, University of California, Los Angeles School of Medicine, USA.
Int J Fertil Menopausal Stud. 1995 Nov-Dec;40(6):322-8.
To assess the effect of varying inseminating sperm concentrations on fertilization rates and polyspermy in human in vitro fertilization (IVF).
Eighty-six couples who completed 107 consecutive IVF cycles were assigned to one of three groups according to the results of their semen analysis (SA), sperm penetration assay (SPA), and titers of antisperm antibodies (ASA). Group 1 (non-male factor) had normal results for SA, SPA and ASA; group 2 had one abnormal result; and group 3 had two or more abnormal results. Inseminating concentrations of 50,000, 250,000, or 500,000 progressively motile sperm/oocyte were prospectively assigned to groups 1, 2 and 3, respectively.
Incidence of polyspermy and fertilization rates.
A total of 992 oocytes were available for analysis. The fertilization rate of 61% for non-male factor patient (group 1) was significantly higher than for male-factor patients [group 2 (48%) and group 3 (43%; P < .01)]. The incidence of polyspermy was 3.3%, 5.5%, and 0% for groups 1, 2 and 3, respectively, and did not differ significantly between the non-male factor and male factor groups (P = .16). Polyspermic fertilization was increased in both mature (4.1%) and postmature (5.7%) as compared to immature oocytes (1.4%; P < .05).
In male factor infertile couples, increasing the inseminating concentration to 250,000 or 500,000 motile sperm/oocyte does not result in an increase in the incidence of polyspermy but does not improve fertilization rates.
评估不同授精精子浓度对人类体外受精（IVF）受精率和多精受精的影响。
86对连续完成107个IVF周期的夫妇根据其精液分析（SA）、精子穿透试验（SPA）和抗精子抗体（ASA）滴度结果被分为三组之一。第1组（非男性因素组）SA、SPA和ASA结果正常；第2组有一项异常结果；第3组有两项或更多异常结果。分别前瞻性地将每卵50,000、250,000或500,000条活动精子的授精浓度分配给第1、2和3组。
多精受精发生率和受精率。
共有992个卵母细胞可供分析。非男性因素患者（第1组）的受精率为61%，显著高于男性因素患者[第2组（48%）和第3组（43%；P<0.01）]。第1、2和3组的多精受精发生率分别为3.3%、5.5%和0%，非男性因素组和男性因素组之间无显著差异（P=0.16）。与未成熟卵母细胞（1.4%）相比，成熟（4.1%）和过成熟（5.7%）卵母细胞的多精受精均增加（P<0.05）。
在男性因素不育夫妇中，将授精浓度提高到每卵250,000或500,000条活动精子不会导致多精受精发生率增加，但也不会提高受精率。
